Isolation and Characterisation of the Corticotropin Releasing Factor Receptor 1 (CRFR1) Gene in a Teleost Fish, Fugu rubripes

Abstract

Corticotropin releasing factor receptor (CRF) is a member of the secretin family of the G-protein coupled receptor superfamily. These are characterised by the presence of seven transmembrane domains and six conserved cysteines that are important for receptor conformation and ligand binding. In vertebrates two CRF receptors (CRF1 and CRF2) have been isolated and characterised. In this study the complete structure of the CRF1 receptor was isolated and partially characterised for the first time in a vertebrate using the compact genome of the Japanese pufferfish, Fugu rubripes as a model. The Fugu CRF1 receptor gene is composed of 14 exons is approximately 27 kb in length. A tissue distribution of this receptor in Fugu reveals that it is expressed mainly in liver, gonads, heart and brain, however, expression in the kidney, gut and gills was also detected. In vertebrates this receptor appears to have a different tissue distribution and its presence in the gills may indicate a new role in osmoregulatory processes.

Keywords::

• Corticotropin releasing factor receptor
• G-protein
• Osmoregulation

Acknowledgements

This work was supported by an MRC grant to M.S.Clark and G.Elgar and a FCT plurianual grant to CCMAR, Portugal. D.M.Power was in receipt of a sabbatical grant (BLS58; FCT, Portugal). J.C.R.Cardoso was funded by FCT, Portugal (grant, BD/19925/99).