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Abstract
Through two-time sequencing randomly in Fenneropenaeus chinensis, 2,597,000 bp cumulative length random genomic sequences about occupying 1.23‰ of the entire genome are obtained, in which the length of the first time sequencing is 8,84,000 bp, by cutting the genome DNA with Sau3AI enzyme, and the second is 1,713,000 bp by breaking the genome DNA with the physical method, ultrasonic. Using tandem repeat finder (TRF) soft to analyze the sequences, 4588 tandem repeats are found, in which the number of microsatellites (1–6 bp) is 3888, and 700 for minisatellites ( ≥ 7 bp). The cumulative length of repeats is 3,05,555 bp, accounting for 11.72% of total cumulative sequence length, in which the cumulative length of microsatellites is 2,32,979 bp, accounting for 8.97% of total sequence length, and greater than those of other organisms, such as human and mosquito, etc. The dinucleotide repeat type is dominant in which the dominant repeat class is AT. The second abundant repeat type is trinucleotide, of which the dominant repeat class is AAT. Interestingly, of all of repeat types, the repeat numbers and repeat classes of primer number repeat types, such as pentanucleotide, heptanucleotide, elevennucleotide, etc. are less than those of repeat types beside them. The phenomena may involve the genesis and the evolution of microsatellites and minisatellites.
Acknowledgements
This work was supported by grants from the National Basic Research Priorities Programme (973)(G1999012007), the National High-Tech Research and Development Program of China (863)(2003AA603021) and Open-end funds of Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences).