Cloning and characterization of farnesyl pyphosphate synthase gene from the ABA-producing fungi Botrytis cinerea

Abstract

Farnesyl pyphosphate synthase (FPPS) catalyzes the systhesis of farnesyl pyphosphate and appears to be a promising regulation site of Abscisic acid (ABA) biosynthesis pathway in fungi. Here we reported the isolation and characterization of Botrytis cinerea (FPPS) gene. The cloned FPPS gene carries an open reading frame of 1044-bp encoding a deduced protein of 347 amino acids with a molecular weight of 39.83 kDa, and the coding region is interrupted with a 63-bp intron. Comparison analysis showed that the deduced amino acids sequence share high similarity with other known FPPS gene. Southern blot revealed a single copy of FPPS gene in the genomic DNA. The result of transcription analysis indicated that the cloned FPPS gene expressed constitutively and was not induced in ABA accumulation phase.

• Farnesyl pyphoshphate synthase
• FPPS
• ABA
• Botrytis cinerea

Acknowledgements

This research was funded by the Key Program of “Eleventh Five-Year” Plan of China and Key Program of “Eleventh Five-Year” Plan of The Chinese Academy of Sciences. We are grateful to Dr Qing-Jie Xia and Dr Wei-Zao Huang of Sichuan University for help in technique assistance.