Nucleotide sequence of a G/11 family xylanase encoding gene in Scytalidium thermophilum

Abstract

The nucleotide sequence of the xylanase encoding gene in Scytalidium thermophilum Af101-3 was determined. The gene encodes a family G/11 xylanase, and the coding region is interrupted by a 72 bp intron. Transcription of the gene was investigated by reverse transcription PCR (RT-PCR). Transcription of the gene was not affected by the presence of 2% glucose in the medium. Xylanase production in S. thermophilum Af101-3 was also affected by concentration of glucose in the medium (modified Czapek's supplemented with 2% corn cob powder and 0.1% glucose). Therefore, xylanase expression in this fungus may not be regulated by the carbon source in the medium.

• Xylanase
• Scytalidium thermophilum
• RT-PCR
• carbon catabolite repression

• AB114442

Acknowledgements

This work was in part supported by collaboration between Yamaguchi University and Kasetsart University in the Core University Program, supported by the Scientific Cooperation Program from the Japanese Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT), and by a grant for Promotion of Education and Research from Yamaguchi University (1998).