Norovirus and sapovirus infections after allogeneic hematopoietic stem cell transplantation: is it worth it to look for them?

Abstract

Norovirus (NoV) and Sapovirus (SaV) are potential causative agents of diarrhea after allogeneic HSCT but little is known in this population. We performed a retrospective analysis by RT-PCR of calicivirus (NoV and SaV), Human adenovirus (HAdV), rotavirus (RV), Aichi virus (AiV), enterovirus (EV), human parechovirus (HPeV) and Human bocavirus (HBoV) in the diarrheal stools of patients after allogeneic HSCT. 49/162 patients had positive viral assays: HAdV (17%), EV (7%), NoV (4.3%), RV and HBoV (3.1% each), SaV (1.9%), AiV (1.2%), HPeV (0.6%). Seven patients were positive for NoV and 3 for SaV. Among viruses-positive samples, the frequency of caliciviruses cases was 7% in the 6 months post-HSCT compared to 40% after (p < 0.0001). The median duration of symptom was 0.7 months but 2 cases, occurring more than one year after HSCT, were chronic, undiagnosed and strongly contributed to morbidity. Systematic testing of caliciviruses appears especially useful in late chronic diarrhea.

Keywords:

• Norovirus
• sapovirus
• diarrhea
• allogeneic hematopoietic stem cell transplantation

Acknowledgments

The authors wish to thank all study participants and the referring clinicians for their valuable contributions.

Author’s contributions

D.R-W. designed the research, L.S., S.N-Q., M.U., A.M. and M.B. took care of the patients, K.A-B. and A.D.R. performed virological analysis, S.B., D.B, K.A-B., A.M. and M.B. collected data, A.M. performed the statistical analysis, A.M, M.B. and D.R-W. wrote the manuscript. All authors reviewed the manuscript.

Disclosure statement

The authors declare no competing interests.

Correction Statement

This article has been corrected with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.