Tert-butylhydroquinone promotes angiogenesis and improves heart functions in rats after myocardial infarction

ABSTRACT

Background: Hypertension is an increased risk of heart failure and acute myocardial infarction (MI). Tert-butylhydroquinone (tBHQ), as an antioxidant, shows multiple cardioprotective actions including the reduction in blood pressure. The aim of this study was to investigate whether and how tBHQ improves heart functions in rats. Methods: The MI model was established in WKY and spontaneously hypertensive rats (SHRs) by ligation of left anterior descending coronary artery. Akt phosphorylation was examined by western blot in human umbilical vein endothelial cells (HUVECs) or in rats. Angiogenesis was assessed by immunohistochemistry and immunofluorescence. Heart function was determined by echocardiography. Results: tBHQ increased Akt phosphorylation, promoted cell proliferations and migrations in HUVECs, which were abolished by Akt inhibitor wortmannin. In SHRs following MI, administration of tBHQ significantly increased Akt phosphorylation, promoted angiogenesis, reduced infarct size, and improved heart functions after 14 postoperative days. Importantly, these in vivo effects of tBHQ were ablated by wortmannin in SHRs. Conclusion: tBHQ via Akt activation promotes ischemia-induced angiogenesis and improves heart functions in hypertensive rats. In perspectives, the application of tBHQ should be considered in patients with ischemic diseases such as MI and stroke.

KEYWORDS:

• Akt
• angiogenesis
• hypertension
• myocardial infarction
• tBHQ

Competing financial interests

The authors declare no competing financial interests.

Additional information

Funding

This work was supported by National Natural Science Foundation of China (81470591, 81570723, and 81673423).

Notes on contributors

Wei-Dong Shi

N.Q.Z. and N.L. designed and performed all experiments. Q.S.P. and W.D.S. convinced the project and wrote the manuscript.