ABSTRACT
This study was conducted to explore the effect of renal denervation (RDN) on the renin–angiotensin–aldosterone system (RAAS) in spontaneously hypertensive rats (SHRs). Our experimental rats were randomly divided into the RDN group conducted by painting 10% phenol on the bilateral renal nerves (RDNX), the shamoperation group simply painting with saline (Sham), and the normotension control group (WKY) following all the animal blood and tissues of kidney, hypothalamus, and adrenal gland collected and examined 2 weeks after RDN operation. We found that the aldosterone (ALD) levels in serum and tissues all decreased in the RDNX group compared with the Sham group (p < .05). Meantime, the expression of angiotensin II type1 receptor (AT1R) mRNA also exhibited significantly reduced by 2.22-fold in the RDNX group compared to the Sham group identical to the expression of AT1R protein in the renal cortex and outer stripe of the outer medulla (OSOM) subjected to denervation surgery, which manifested the lower ATIR protein expression than the Sham group (p < .05). Besides, the expression of angiotensin II (Ang II) protein in the cortex , OSOM, and inner stripe of the outer medulla were all attenuated by RDN in comparison with the Sham group (p < .05). RDN reduced intrarenal RAAS and circulating RAAS to lower blood pressure and repair renal function.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Grant statement
This work was supported by the National Natural Science Foundation of China under Grant Number [81660075], the Postdoctoral Research Foundation of China under Grant Number [2017M612872], the Opening Project of the Science Experiment Center, Guangxi under Grant Number [KFJJ2011-35], and the research funding from Guangxi Provincial Education Office under Grant Number [201202ZD025]. The funders had no role in the study design, data collection, analysis, interpretation of the data, decision to submit results, the decision to publish, or preparation of the manuscript.
Ethical approval
These protocols completely conformed to the relevant ethical standards of Regulation for the Administration of Affairs Concerning Experiment Animals (proved by the State Council on October 31, 1988, and promulgated by Decree No. 2 of the State Science and Technology Commission on November 14, 1988) and were approved by the committees on animal research of Guangxi Medical University (China).