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Abstract
The free radical, nitric oxide (√NO), is responsible for a myriad of physiological functions. The ability to verify and study √NO in vivo is required to provide insight into the events taking place upon its generation and in particular the flux of √NO at relevant cellular sites. With this in mind, several iron-chelates (Fe2+(L)2) have been developed, which have provided a useful tool for the study and identification of √NO through spin-trapping and electron paramagnetic resonance (EPR) spectroscopy. However, the effectiveness of √NO detection is dependent on the Fe2+(L)2 complex. The development of more efficient and stable Fe2+(L)2 chelates may help to better understand the role of √NO in vivo. In this paper, we present data comparing several proline derived iron–dithiocarbamate complexes with the more commonly used spin traps for √NO, Fe2+-di(N-methyl-D-glutamine-dithiocarbamate) (Fe2+(MGD)2) and Fe2+-di(N-(dithiocarboxy)sarcosine) (Fe2+(DTCS)2). We evaluate the apparent rate constant (kapp) for the reaction of √NO with these Fe2+(L)2 complexes and the stability of the corresponding Fe2+(NO)(L)2 in presence of NOS I.
| Abbreviations | ||
| EPR | = | electron paramagnetic resonance |
| NOS | = | nitric oxide synthase |
| Fe2+(DETC)2 | = | Fe2+-di(N,N-diethyldithiocarbamate) |
| Fe2+(MGD)2 | = | Fe2+-di(N-methyl-D-glutaminedithiocarbamate) |
| Fe2+(DTCS)2 | = | Fe2+-di(N-(dithiocarboxy)sarcosine) |
| Fe2+(DTCP)2 | = | Fe2+-di(N-(dithiocarboxy)-L-proline) |
| Fe2+(DTCHP)2 | = | Fe2+-di(N-(dithiocarboxy)-trans-4-hydroxy-L-proline) |
| Fe2+(MSD)2 | = | Fe2+-di(N-(dithiocarboxy)-N-methyl-L-serine) |
| Hb(Fe2+)O2 | = | oxyhemoglobin |
| Abbreviations | ||
| EPR | = | electron paramagnetic resonance |
| NOS | = | nitric oxide synthase |
| Fe2+(DETC)2 | = | Fe2+-di(N,N-diethyldithiocarbamate) |
| Fe2+(MGD)2 | = | Fe2+-di(N-methyl-D-glutaminedithiocarbamate) |
| Fe2+(DTCS)2 | = | Fe2+-di(N-(dithiocarboxy)sarcosine) |
| Fe2+(DTCP)2 | = | Fe2+-di(N-(dithiocarboxy)-L-proline) |
| Fe2+(DTCHP)2 | = | Fe2+-di(N-(dithiocarboxy)-trans-4-hydroxy-L-proline) |
| Fe2+(MSD)2 | = | Fe2+-di(N-(dithiocarboxy)-N-methyl-L-serine) |
| Hb(Fe2+)O2 | = | oxyhemoglobin |