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Abstract
Intracellular reactive oxygen species (ROS) were attenuated by the expression of peroxiredoxin II (Prx II). Cellular senescence as judged by senescence-associated (SA)-β-galactosidase (Gal) positive cell formation was increased in Prx II-deficient mouse embryonic fibroblast (MEF). Ras expression was increased following passages. The level of Ras expression was higher in Prx II− / − MEF than wild type MEF. ERK activity was also augmented by the deletion of Prx II. SA-β-Gal-positive cell formation was reduced by PD98059, ERK inhibitor. Activated nuclear transcription factor, nuclear factor-kappaB (NFκB) by the deletion of Prx II was inhibited by the treatment with PD98059. In contrast, no changes in SA-β-Gal-positive cell formation were detected by NFκB inhibitor, N-alpha-tosyl-l-phenylalanyl chloromethyl ketone (TPCK). Collectively, results suggest that Prx II deletion activate Ras–ERK–NFκB pathways and cellular senescence in Prx II− / − MEF cells was mediated by ERK activation but not by NFκB activation.
| Abbreviations | ||
| ROS | = | reactive oxygen species |
| ERK | = | extracellular receptor kinase |
| MEF | = | mouse embryonic fibroblast |
| Prx II | = | peroxiredoxin II |
| SA-β-Gal | = | senescence-associated-β-glactosidase |
| SOD | = | superoxide dismutase |
| SEAP | = | secreted alkaline phosphatase |
| MBP | = | myelin basic protein |
| PMA | = | phorbol 12-myristate 13-acetate |
| TPCK | = | N-alpha-tosyl-l-phenylalanyl chloromethyl ketone |
| NFκB | = | nuclear factor kappaB |
| EMSA | = | electrophoretic mobility shift assay |
| IOM | = | ionomycin |
| Abbreviations | ||
| ROS | = | reactive oxygen species |
| ERK | = | extracellular receptor kinase |
| MEF | = | mouse embryonic fibroblast |
| Prx II | = | peroxiredoxin II |
| SA-β-Gal | = | senescence-associated-β-glactosidase |
| SOD | = | superoxide dismutase |
| SEAP | = | secreted alkaline phosphatase |
| MBP | = | myelin basic protein |
| PMA | = | phorbol 12-myristate 13-acetate |
| TPCK | = | N-alpha-tosyl-l-phenylalanyl chloromethyl ketone |
| NFκB | = | nuclear factor kappaB |
| EMSA | = | electrophoretic mobility shift assay |
| IOM | = | ionomycin |