Lactobacillus brevis MTCC 1750 enhances oxidative stress resistance and lifespan extension with improved physiological and functional capacity in Caenorhabditis elegans via the DAF-16 pathway

Abstract

Redox imbalance plays a crucial role in the development of age-related diseases, and resistance to oxidative stress is crucial for optimum longevity and healthy aging. Using the wild-type, mutant and transgenic strains, this study explored the antioxidative potential and lifespan extension benefits of different Lactobacillus strains in Caenorhabditis elegans (C. elegans). We observed that Lactobacillus brevis MTCC 1750 could enhance the resistance of C. elegans against juglone induced oxidative stress by reducing its intracellular reactive oxygen species (ROS) accumulation. Also, live L. brevis MTCC 1750 could prolong the worm’s lifespan. These effects are dependent on transcription factor DAF-16 evident with significant upregulation of its target gene sod-3. This also explained the significant improvements in different age-associated changes in physiological and mechanical parameters of the worm by L. brevis MTCC 1750. Further investigations revealed that DAF-16 activation and, its enhanced translocation in the nucleus is independent of DAF-2 or JNK pathway. These findings highlighted L. brevis MTCC 1750 as a potent anti-oxidant source for complementing current antioxidant therapeutic strategies. Nonetheless, the findings showed how different signaling events are regulated based on an organism’s diet component, and their consequences on the aging process in multiple species.

Keywords:

• Lactobacillus brevis MTCC 1750
• Caenorhabditis elegans
• longevity
• oxidative stress
• DAF-16

Acknowledgements

The authors acknowledge the Department of Biochemistry and Molecular Biology, Pondicherry University, India, Department of Science & Technology (DST), Science and Engineering Research Board (SERB), Ministry of Tribal Affairs, Government of India, University Grants Commission (UGC), New Delhi, India.

Author contributions

SK: Conceptualization, methodology, experiments, investigation, formal analysis, writing original draft, editing. NSP: formal analysis, writing draft, experiments. KS: Supervision, conceptualization, validation, analysis, review and editing, funding acquisition.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Data will be made available on request.

Additional information

Funding

This work was supported by the Department of Biochemistry and Molecular Biology, Pondicherry University, India. SK is a recipient of National Fellowship, Government of India (Award No − 201920-NFST-DEL-01172).