Article title: Radiotherapy-induced enrichment of EGF-modified doxorubicin nanoparticles enhances the therapeutic outcome of lung cancer
Authors: Jing Wang, Yan Zhang, GuangPeng Zhang, Li Xiang, HaoWen Pang, Kang Xiong, Yun Lu, JianMei Li, Jie Dai, Sheng Lin and ShaoZhi Fu
Journal: Drug Delivery
Bibliometrics: Volume 29, Number 1, pages 588–599
DOI: https://doi.org/10.1080/10717544.2022.2036871
When this article was first published online, the following contents and figures were typeset incorrectly.
Section 2.1. Synthesis and characterization of PEI-PLA-PEG-PLAPEI copolymer has been corrected as below:
To synthesize the copolymer PEI-PLA-PEG-PLAPEI (PELI), 1 mM PELA was first dissolved in 40 mL dichloromethane, then 1mM 4-(dimethylamino) pyridine (DMAP) and 3 mM succinic anhydride was added to the PELA solution, the reaction was carried out at room temperature for 24 h under nitrogen atmosphere (N2). The product was precipitated with pre-cooled petroleum ether, vacuum-dried, and 1 mM HOOC-PELA-COOH copolymer, 1mM N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), 1 mM N-Hydroxy-succinimide (NHS) was dissolved in 20 mL chloroform for activation 2 h. Finally, 2 mM PEI solution in methanol was slowly added, and the reaction was performed at RT for 24 h under N2. The final product was precipitated in petroleum ether twice, dried in a vacuum oven at room temperature
and were incorrect and this been corrected with the below mentioned revised figures.
Figure 4. In vitro cell uptake. (A) Fluorescence microscopy images showing the uptake of DOX NPs into the EGFRhigh A549 cells, and the EGFRlow AML-12 and BEAS-2B cells. (DOX concentration: 2.5 μg/mL, EGF pretreatment for 4 h in the EGFþEGF@DOX-NPs group; Scale bar: 100 μm). (B–D) DOX content in the A549, AML-12, and BEAS-2B cells was determined by flow cytometry. (E) EGFR protein expression in the A549, AML-12, and BEAS-2B cells was assessed by Western blot analysis. β-Actin expression served as a loading control.
Figure 5. Typical images of wound healing of A549 monolayer after treatment with different drugs for 0, 6, 12, and 24 h (DOX concentration: 1 μg/mL; Scale bar: 500 μm).
Now, the article has been republished online.