Fabrication and in vitro evaluation of thermally cross-linked gelatin nanofibers for drug delivery applications

Abstract

In this study, four different nanofibers consisting of gelatin (Gel), doxorubicin (DOX) with gel (DOX@Gel), a composite of gel with poly(ethylene glycol) (PEGylated-gel), and DOX@PEGylated-gel were fabricated. Subsequently, the nanofibers were thermally cross-linked in order to offer a stable and biocompatible alternative for the biological applications of nanofibers such as drug delivery and tissue engineering. Nanofibers were characterized by scanning electron microscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), and confocal microscopy. The formation of smooth, continuous, and uniform nanofibers was observed and the addition of PEG resulted in an increase whereas the incorporation of DOX into nanofibers had no significant change in the diameter of nanofibers. Crosslinking also enlarged the diameter of all nanofibers and the most dramatic increase was observed 53% by DOX@PEGylated-gel. Afterward, the biological performance of the nanofibers was investigated by drug release profile, cytotoxicity on A549 cell line as well as antimicrobial activity with E. coli and S. aureus. The results indicate an enhanced drug release profile, moderate antimicrobial activity, and reasonable cytotoxic efficiency for thermally cross-linked nanofibers compared to uncross-linked nanofibers.

Graphical Abstract

Keywords:

• Electrospinning
• drug delivery
• gelatin
• nanofiber
• stability
• thermal crosslinking

Acknowledgments

The authors would like to thank the Center of Material Research and Biotechnology & Bioengineering Research Center at İzmir Institute of Technology for their help and technical support.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Informed Consent

All authors are consent to publish the manuscript.

Authors’ contributions

D.M.: research, methodology, investigation, data collection, analysis, writing - original draft. G.G.: data collection, analysis. G.Ş.M.: research conceptualization, supervision, project administration, writing-review, and editing.