Metabonomics Study of the Anti-Osteoporosis Effect of Velvet Collagen Hydrolysate Using Rapid Resolution Liquid Chromatography Combined with Quadrupole Time-of-Flight Tandem Mass Spectrometry

Abstract

A metabonomics study using RRLC-Q-TOF/MS was performed to obtain a systematic view of the osteoporosis and anti-osteoporosis effects of velvet collagen hydrolysate. Combined with multivariate statistics, the urinary difference was determined between the model group and control group and between the treatment group and model group. Biochemical and pathway analyses were also performed to validate the osteoporosis model and investigate the anti-osteoporosis effect of velvet collagen hydrolysate. A clear separation trend was observed in the PCA scores plot. A total of five compounds were identified as significantly changed metabolites in the urine samples and may be potential biomarkers. This study demonstrates that the action mechanism of velvet collagen hydrolysate on osteoporosis may involve the regulation of amino acid metabolism and phospholipid metabolism.

Keywords:

• metabonomics
• osteoporosis
• Principal Component Analysis
• RRLC-Q-TOF/MS
• velvet collagen hydrolysate

Acknowledgment

We thank Prof. Yu Zhao and Daqing Zhao (Changchun University of Chinese Medicine) for graciously donating velvet collagen hydrolysate.

Notes

Na Li and Lijing Zhao contributed equally to this work.