Elucidation of molecular diversity and functional characterization of phenanthrene degrading consortium NS-PAH-2015-PNP-5

Abstract

Phenanthrene (PHE) is a hazardous low molecular weight PAH and widespread ecological contaminant. In the present study, the Illumina Miseq platform explored the bacterial diversity of the efficient PHE degrading consortium NS-PAH-2015-PNP-5, developed from the petrochemical contaminated soil near the Indian Oil Corporation Limited refinery, Panipat (India). Total 273 OTUs represented the species diversity identified in NS-PAH-2015-PNP-5. Nineteen major bacterial species covered approximately 99.22% of the total α-diversity of NS-PAH-2015-PNP-5. In NS-PAH-2015-PNP-5, the Shannon alpha diversity (H') and Pielou's Evenness Index (J') were calculated at 2.43 and 0.43, respectively. Moreover, an overwhelming population of phylum Proteobacteria (99.28%) and Pseudomonas spp. (32.25%) were observed in NS-PAH-2015-PNP-5. Two culturable isolates, PAH 51 and PAH 52, were isolated from the NS-PAH-2015-PNP-5, and the result of 16S rRNA analysis showed homology with Bacillus wiedmannii (96.7%) and Achromobacter pulmonis (99.24%), respectively. The results of the carbohydrate utilization test showed that NS-PAH-2015-PNP-5 was able to metabolize dextrose, L-arabinose, ONPG, esculin, citrate, and malonate. Detection of phthalic acid metabolite by the GCMS analysis inferred that NS-PAH-2015-PNP-5 bio-degraded the PHE through the protocatechuic acid pathway. During the seven-day biodegradation experiment at the optimized physicochemical conditions, NS-PAH-2015-PNP-5 degraded 62%, 70%, 90% PHE after the third, fifth, and seventh day, respectively.

Graphical Abstract

1. A foremost population of phylum Proteobacteria was identified in NS-PAH-2015-PNP-5.

2. The culturable isolates of NS-PAH-2015-PNP-5 (PAH 51 and PAH 52) didn't utilize PHE as the carbon source.

3. Biodegradation of PHE by the NS-PAH-2015-PNP-5 was accomplished through the protocatechuic acid pathway.

4. Pseudomonas spp. were the most abundant species of the total α-diversity of NS-PAH-2015-PNP-5.

Keywords:

• Alpha diversity
• biodegradation
• consortium
• operational taxonomic unit
• phenanthrene
• taxon

Acknowledgements

The authors are grateful to DST-PURSE, DST Indo-Ukraine Project, HSCST (Panchkula), UGC-SAP, BIF-DBT, DST-FIST for the financial support of the project.

Disclosure statement

There is no conflict of interest among the authors.