Serum levels and ApaI polymorphism of insulin-like growth factor 2 on intrauterine growth restriction infants

Abstract

Purpose: The aim of our study was to evaluate the IGF2 and IGF2R plasmatic level and IGF2-ApaI polymorphism on infants with intrauterine growth restriction (IUGR).

Materials and methods: A transversal study was conducted at the Neonatology Ward of the Gynecology Clinic I, Emergency Hospital Cluj-Napoca on neonates with IUGR who were discharged during June 2014 and June 2015. The serum levels of IGF2 and IGF2R were obtained by using ELISA method and IGF2-ApaI polymorphism by taking PCR-RFLP analysis.

Results: Forty infants with IUGR and 21 infants of appropriate gestational age (AGA) were evaluated. The serum levels of IGF2 proved higher on the A/G genotype when the IUGR group was compared with AGA (p value = .048). The G allele proved significantly more frequent in both the IUGR and the AGA group compared with the A allele (p < .002). Neither any allele nor any genotype proved a risk factor for IUGR (p value > .3). The A/G genotype proved significantly more frequent on term infants compared with preterm infants (p value = .039).

Conclusions: The infant with IUGR has a higher serum level of IGF2 if has A/G IGF2-ApaI genotype and higher values of IGF2R if it has the A/A genotype.

Keywords:

• Intrauterine growth restriction (IUGR)
• insulin-like growth factor 2 (IGF2)
• insulin-like growth factor 2 receptor (IGF2R)
• single nucleotide polymorphisms (SNPs)

Acknowledgements

The authors thank to Mrs. Manuela Sfichi, PhD, and Sanda Bursasiu for the excellent technical assistance. This paper was published under the frame of European Social Found, Human Resources Development Operational Programme 2007–2013, Project no. POSDRU/159/1.5/S/138776.

Disclosure statement

The authors report no conflicts of interest.

Additional information

Funding

This paper was published under the frame of European Social Found, Human Resources Development Operational Programme 2007–2013, Project no. POSDRU/159/1.5/S/138776.