Association of SP-B gene 9306 A/G polymorphism (rs7316) and risk of RDS

Abstract

Background: Respiratory distress syndrome (RDS) is a severe pulmonary disease predominantly affects preterm newborns. Polymorphisms of surfactant-protein genes have been mostly evaluated as the candidate contributors in genetics of RDS. However the results are divers in different studies. We aimed at investigating the association of surfactant protein B (SPB) gene 9306 A/G polymorphism (rs7316) with RDS development.

Method: Three hundred and eighty newborns with gestational age of less than 34 weeks were included in a multicenter case–control study. Respiratory distress (RD) was scored according to Downes’ scoring system. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used for genotyping.

Result: One hundred and eighty-four neonates showed RDS and 196 did not. Gestational age (GA) was significantly lower in the RDS group compared with the controls. AA genotype and A allele were found more frequently in the RDS group than the controls (96.2% versus 63.8% and 98.1% versus 80.6%, respectively) (p =.0001).

Conclusions: This is the first report of association of SFTPB rs7316 polymorphism with RDS development in Iranian newborns. The current study suggests that GA <28-weeks is the most important factor in predisposition to RDS. Genetic background in terms of SP-B gene might be involved in predisposition to RDS in premature neonates.

Keywords:

• Iran
• RDS
• rs7316
• SFTPB
• SP-B 9306

Acknowledgements

The authors are thankful to the all participants and their parents who have shown enthusiasm towards this project. The authors are grateful to the all nurses and other medical staffs who helped us in this project.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This study was supported by a grant from Tehran University of Medical Sciences and Health Services (Grant no. 92-01-91-21624).