Antimicrobial flavonoids from Tridax procumbens

Abstract

Callus culture of Tridax procumbens has been established on Murashige and Skoog's medium supplemented with NAA and BAP from nodal segments. Free and bound flavonoids were extracted from 2, 4, 6 and 8 weeks old calli by a well-established method. These free flavonoids were then screened against Staphylococcus aureus (bacteria) and Candida albicans (yeast) for their antimicrobial potential. Minimum inhibitory concentration, minimum bactericidal/fungicidal concentrations and total activity were also evaluated. Apigenin, quercetin and kaempferol were identified from free flavonoids of 4 weeks old callus (most active) through, thin layer chromatography, (TLC) preparative TLC, MP and IR spectral studies.

Keywords:

• T. procumbens
• callus
• flavonoid
• antimicrobial potential

Acknowledgements

The authors are grateful to the Head, Department of Botany, University of Rajasthan, Jaipur, India, for providing all the facilities to carry out the work.