Cytotoxicity and Synergistic Effect of the Constituents from Roots of Aglaia odorata (Meliaceae)

Abstract

Twelve compounds were isolated from the roots of Aglaia odorata. Their structures were established on the basis of NMR and MS data as rocaglaol (1), rocaglamide (2), eichlerialactone (3), sapelins A (4), isofouquierone (5), eichlerianic acid (6), shoreic acid (7), agladupol E (8), 3-epimeliantriol (9), cleomiscosins B (10), 2β,3β-dihydroxy-5α-pregnane-16-one (11) and β-d-glucopyranos-1-yl N-methylpyrrole-2-carboxylate (12). Among them, compounds 1 and 2 showed significant cytotoxicity against human cancer cell (HL-60, SMMC-7721, A-549, MCF-7 and SW480) with IC₅₀ values of 0.007–0.095 μM, while compounds 3–5 and 10 and 11 showed moderate to no cytotoxicity (IC₅₀ 0.43 to values >40 μM). Compound 6 showed only weak cytotoxicity (IC₅₀ 6.87 to >40 μM) and its epmier 7 was completely inactivite (IC₅₀>40 μM) in the assay. However, potent synergistic effect was observed when the molar ratio of 6 to 7 is between 4:1 and 1:1.

Keywords:

• Aglaia odorata
• cytotoxicity
• flavaglines
• epimer
• synergistic effect

Additional information

Funding

This work was supported by the Chinese Academy of Sciences (CAS) 135 program [grant number XTBG-F02]; National Natural Science Foundation of China [grant number NSFC-U1302222].