Protective effects of alpha-lipoic acid on diazinon-induced renal toxicity in rats: an immunohistochemistry study

Abstract

The current study aimed to examine the impacts of alpha-lipoic acid (ALA) on oxidative stress parameters and the expression of fibronectin and laminin in the renal corpuscle of male Wistar rats following exposure to diazinon (DZN). The animals were distributed into the following groups: control, DZN (40 mg/kg), DZN + ALA (40 mg/kg + 100 mg/kg), ALA (100 mg/kg), and sham. Diazinon induced lipid peroxidation and oxidative stress and reduced the distribution and mRNA expression of laminin and fibronectin. ALA reduces the nephrotoxic effects of diazinon. In conclusion, ALA plays a therapeutic role in the treatment of oxidative stress-associated toxicities and diseases.

Keywords:

• Alpha-lipoic acid
• diazinon
• fibronectin
• imageJ
• immunohistochemistry
• in vivo
• kidney
• laminin
• mRNA expression
• oxidative stress

Acknowledgements

The authors express their appreciation to Mrs. Amini, Miss Jaberi, Mr. Delavar, and Mr. Jalili-Nik for their technical assistance.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The current study was extracted from the Ph.D. Thesis of Fatemeh Rahimi Anbarkeh. The Deputy Research of Mashhad University of Medical Science funded this project under the contract [NO. 951735].