Downregulation of lncRNAs Gomafu, NONMMUT033604.2, and NONMMUT064397.2 in the hippocampus of mice with model of post-traumatic stress disorder

Abstract

Objectives

Molecular mechanisms of post-traumatic stress disorder (PTSD) development have been analysed by evaluati-ng changes in the expression level of long non-coding RNA (lncRNA) as a potential biomarker of the disease and as one of the molecular aspects associated with the disease development.

Methods

In our study, we used quantitative polymerase chain reaction (qPCR) to evaluate changes in the expression level of long non-coding RNA – Gomafu, NONMMUT033604.2, and NONMMUT064397.2 – in the hippocampus of mice that were subjected to an artificially induced middle single prolonged stress (mSPS) model of post-traumatic stress disorder.

Results

We found a significant reduction in the expression levels of each of the three lncRNAs tested: Gomafu in 45.4 times, NONMMUT033604.2 in 53.4 times, and NONMMUT064397.2 in 5.2 times. The results of the present study provide evidence that the mSPS model effectively induces PTSD-like behaviour in mice leading to a significant decrease in the expression level of Gomafu, NONMMUT033604.2 and NONMMUT064397.2 lncRNA in mice hippocampus.

Conclusions

This data provides evidence that the three studied lncRNAs could be potential biomarkers of PTSD development.

Keywords:

• PTSD
• lncRNA
• hippocampus

Acknowledgements

N/A

Authors contributions

Klymenko A.: conceptualisation, methodology, investigation, writing- original draft preparation

Nagibin V.: formal analysis, writing - review & editing

Gorlova A.: investigation, writing - review & editing

Dobropolska Y.: methodology, investigation, writing - review & editing

Bohovyk R.: software

Isaev D. supervision, validation, formal analysis

Dosenko V.: validation, formal analysis, resources, supervision

Ethical statement

All animal experiments complied with the ARRIVE guidelines and were approved by the local Animal Ethics Committee of Bogomoletz Institute of Physiology (Kyiv, Ukraine) and were held in accordance with the European Commission Directive (2010/63/EU for animal experiments).

Statement of interest

None to declare.

Additional information

Funding

No funding sources was involved in this investigation.