Overview on biological implications of metal oxide nanoparticle exposure to human alveolar A549 cell line

Abstract

Metal oxides (MeOx) are exponentially being used in a wide range of applications and are the largest class of commercially produced nanomaterials. This presents unprecedented human exposure. Thus, understanding nanoparticle induced cellular stress can greatly help design strategies to combat them. Scores of studies have been carried out to understand the effects of MeOx nanoparticle exposure on human alveolar cells, which are highly susceptible to aerosolized matter. There is a huge redundancy of information generated, also, a lack of a comprehensive conglomeration of this information. We have built here in a sincere summary of the cellular responses reported till date as a direct consequence of MeOx nanoparticle exposure on human alveolar (A549) cells. Detailed accounts of cellular morphology modulation, generation of reactive oxygen species (ROS) and oxidative stress, inflammation and cytokine release, genotoxic and epi-genotoxic insults, toxicological trend, nanoparticle internalization, modes of cell death, protein synthesis, and membrane damage among others are discussed. Finally, to aid predictability of the highly dynamic and multifactorial nature of this toxicity, we have hypothesized models that describe the ensuing mechanisms based on common patterns discovered throughout our literature survey.

Keywords:

• Metal oxide
• nanoparticles
• A549
• ROS
• toxicity
• DNA damage
• internalization
• mechanism
• signaling
• alveolar cells

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

BRNS10.13039/5011000065932011/37B/25/BRNS/1252

DBT10.13039/5011000014076242-P59/RGCB/PMD/DBT/ANSR/2015

BITS Pilani10.13039/501100006464

AS would like to acknowledge BRNS (2011/37B/25/BRNS/1252) and DBT 6242-P59/RGCB/PMD/DBT/ANSR/2015 Govt. of India for research funding. AM is supported by BITS Pilani for her research fellowship.