Superparamagnetic iron oxide nanoparticles (SPIONs) modulate hERG ion channel activity

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used in various biomedical applications, such as diagnostic agents in magnetic resonance imaging (MRI), for drug delivery vehicles and in hyperthermia treatment of tumors. Although the potential benefits of SPIONs are considerable, there is a distinct need to identify any potential cellular damage associated with their use. Since human ether à go-go-related gene (hERG) channel, a protein involved in the repolarization phase of cardiac action potential, is considered one of the main targets in the drug discovery process, we decided to evaluate the effects of SPIONs on hERG channel activity and to determine whether the oxidation state, the dimensions and the coating of nanoparticles (NPs) can influence the interaction with hERG channel. Using patch clamp recordings, we found that SPIONs inhibit hERG current and this effect depends on the coating of NPs. In particular, SPIONs with covalent coating aminopropylphosphonic acid (APPA) have a milder effect on hERG activity. We observed that the time-course of hERG channel modulation by SPIONs is biphasic, with a transient increase (∼20% of the amplitude) occurring within the first 1–3 min of perfusion of NPs, followed by a slower inhibition. Moreover, in the presence of SPIONs, deactivation kinetics accelerated and the activation and inactivation I–V curves were right-shifted, similarly to the effect described for the binding of other divalent metal ions (e.g. Cd²⁺ and Zn²⁺). Finally, our data show that a bigger size and the complete oxidation of SPIONs can significantly decrease hERG channel inhibition. Taken together, these results support the view that Fe²⁺ ions released from magnetite NPs may represent a cardiac risk factor, since they alter hERG gating and these alterations could compromise the cardiac action potential.

Keywords:

• SPIONs
• hERG channel
• cardiotoxicity
• iron nanoparticles

Acknowledgments

The authors acknowledge Michele Petrecca for the help to record FTIR spectra and Martin Albino for ICP-MS measurements. Patch-clamp measurements were performed at the RISE A-MeProLab of the Department of Chemistry ‘Ugo Schiff’ (University of Florence).

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

Financial support from Fondazione Cassa di Risparmio di Firenze and FAS (Fondo Aree Sottoutilizzate) 2007–2013 project are gratefully acknowledged.