ABSTRACT
Sudan dyes were investigated in branded and non-branded spices, commonly available in the markets of Karachi, Pakistan. High performance liquid chromatography (HPLC) with a variable wavelength detector (VWD) was applied to determine Sudan dyes I-IV. The non-branded samples had higher concentrations of Sudan dyes than the maximum limits of 0.1 mg/kg. The highest concentration of Sudan dye (I) was found in turmeric powder (8460 mg/kg) and the lowest concentration (1.50 mg/kg) of Sudan (IV) in Chaat Masala. This indicates that the use of non-branded spices is not safe, whereas no Sudan dye was found in the branded spice samples. Further studies regarding the higher carcinogenic risk posed by Sudan dye adulterated spices in Pakistan is strongly advised.
Acknowledgements
The authors are extremely grateful to Dr. Peter J. Baugh for reviewing and improving the linguistic quality of this manuscript. Peter J. Baugh is from The British Mass Spectrometry Society (BMSS), C/O 23, Priory Road, Sale, M33 2BU, England, UK.
Disclosure statement
No potential conflict of interest was reported by the authors.