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Articles

Isolation and screening of microalgae from agro-industrial wastewater (POME) for biomass and biodiesel sources

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Pages 29118-29125 | Received 02 Sep 2015, Accepted 04 Dec 2015, Published online: 05 Feb 2016
 

Abstract

Indigenous microalgae (KR349061) were isolated from the agro-industrial wastewater and screened in order to determine their potential for biomass and biodiesel sources. Bold’s Basal Medium was used to isolate the colonies from sample. The nutrient contents were altered in order to optimize the growth of the microalgae. The isolates were identified based on the morphology and microscopic appearance of the isolated colonies. It was seen that the isolates comprised of several common green microalgae and cyanobacteria, among which the ideal candidate was selected and further investigated. Lipid content was determined for the strains that showed rapid growth. The results showed that Chlamydomonas incerta achieved highest lipid content of biomass (42.6%) after 2 d, also highest lipid productivity of 0.197 mg/L/d was observed when the initial COD concentration was 500 mg/L. C. incerta also showed higher growth rate and higher amount of lipid was examined for its potential as sustainable biodiesel feedstock along with its higher biomass yield.

Acknowledgments

The authors would like to acknowledge CRC and IPASA, Universiti Teknologi Malaysia for providing adequate facilities to conduct this research and for their financial supports of this research through the Research University Grant and MOSTI fund with Vot No. 4S042.

Notes

Presented at The 2nd IWA Malaysia Young Water Professionals Conference 2015 (YWP15) Aspiring Beyond Water Solution, March 17–20, 2015, Langkawi Island, Malaysia

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