Publication Cover
Nutritional Neuroscience
An International Journal on Nutrition, Diet and Nervous System
Volume 18, 2015 - Issue 3
135
Views
25
CrossRef citations to date
0
Altmetric
Original Research Papers

Mechanism of protective effect of lettuce against glucose/serum deprivation-induced neurotoxicity

, &
Pages 103-109 | Published online: 15 Jan 2014
 

Abstract

Objectives

The present study investigated the protective effect of ethyl acetate fraction of lettuce (Lactuca sativa) against glucose/serum deprivation (GSD)-induced neurotoxicity, a model which simulates neuronal damage during ischemia.

Methods

Two neuron-like cells, N2a and PC12, were cultivated for 12 hours in GSD condition in the absence or presence of the lettuce fraction. The cell viability, DNA damage, and proapoptotic or antiapoptotic proteins levels were determined using MTT, comet, and immunoblotting assays, respectively. In addition, the intracellular reactive oxygen species and lipid peroxidation levels were measured by fluorimetric methods.

Results

In both N2a and PC12 cells, GSD condition significantly decreased the cell viability which was accompanied by increased intracellular reactive oxygen species production, lipid peroxidation level, and oxidative DNA damage. All the GSD-induced neurotoxic changes were inhibited by the lettuce fraction. Lettuce also suppressed the elevated Bax and caspase-3 proteins and decreased Bcl-2 induced by GSD in PC12 cells.

Discussion

The present study revealed that lettuce exerts neuroprotective effect through decrease of oxidative stress and inhibition of proapoptotic pathways. Therefore, it has the potential to be used for the management of ischemia-induced neuronal damage.

Acknowledgements

This work was supported by a grant from Research Council of Mashhad University of Medical Sciences, Mashhad, Iran. The authors declare that there are no conflicts of interest.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 273.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.