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Abstract
Objectives: Bronchopulmonary dysplasia (BPD) is a significant global health problem and currently lacks effective therapy. We established a neonatal rat model of BPD to investigate therapeutic potential of bone marrow-derived mesenchymal stem cells (BMSCs) in neonatal hyperoxic lung injury.
Methods: BMSCs were isolated, identified, and transfected by lentiviral vector carrying green fluorescent protein gene in vitro. Neonatal Sprague-Dawley rats were injected intravenously with either BMSCs or phosphate-buffered saline following 95% oxygen exposure, and assessed for the survival rate and alveolar injury during recovery.
Results: Treatment with BMSCs after oxygen exposure for 7 days improved survival of neonatal rat during recovery. BMSCs protected against neonatal rat hyperoxic lung injury during recovery as demonstrated by enhanced expression of AQP5 and SP-C, likely due to the suppression of alveolar cell apoptosis and lung inflammation responses to oxygen with up-regulation of the expression of BCL-2 gene and down-regulation of the expression of BAX gene and stimulation of vascular endothelial growth factor and so on.
Conclusions: BMSCs protect against O2-mediated injury partially through stimulation of potent mediators that participate in tissue repair.
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Acknowledgements
We thank Cyagen Biosciences, Guangzhou, China, for the construction of lentiviral vector (pLV/EX2D-Neo-EF1AeGFP). H Zhang contributed in an important manner to the study design and performance, data collection and analysis, and writing of the manuscript. J Fang conceived of the study, and participated in its design and coordination and helped to draft the manuscript. Y Wu participated in animal experiments and carried out the immunoassays. Y Mai participated in animal experiments. W Lai participated in BMSCs harvest and cell culture. H Su participated in the design of the study and performed the statistical analysis.