Bone formation in calvarial defects by injectable nanoparticular scaffold loaded with stem cells

Abstract

Objective: Calcium phosphates are one of biomaterials that are used for bone regeneration. In this study, calcium phosphate nanoparticles such as hydroxyapatite (HA)/fluorapatite (FA),with chitosan gel filled with unrestricted somatic stem cells (USSCs) were used for healing calvarial bone in rat model.

Methods: The healing effects of these injectable scaffolds, with and without stem cells, in bone regeneration were investigated by computed tomography (CT) analysis and pathology assays after 28 days of grafting.

Results: The results of CT analysis showed that bone regeneration on the scaffolds, and the amounts of regenerated new bone for USSC scaffold were significantly greater than the scaffold without cell and untreated controls.

Conclusion: Therefore, the combination of scaffold especially with USSC could be considered as a useful method for bone regeneration.

Keywords::

• bone regeneration
• calcium phosphate nanoparticles
• chitosan
• Hydroxyapatite/Flouroapatite
• injectable gel
• unrestricted somatic stem cell

Declaration of interest

The authors state no conflict of interest and have received no payment in preparation of this manuscript.

Notes