Cancer gene therapy with targeted adenoviruses

Abstract

Background: Clinical experience with adenovirus vectors has highlighted the need for improved delivery and targeting. Objective: This manuscript aims to provide an overview of the techniques currently under development for improving adenovirus delivery to malignant cells in vivo. Methods: Primary research articles reporting improvements in adenoviral gene delivery are described. Strategies include genetic modification of viral coat proteins, non-genetic modifications including polymer encapsulation approaches and pharmacological interventions. Results/conclusion: Reprogramming adenovirus tropism in vitro has been convincingly demonstrated using a range of genetic and physical strategies. These studies have provided new insights into our understanding of virology and the field is progressing. However, there are still some limitations that need special consideration before adenovirus-targeted cancer gene therapy emerges as a routine treatment in the clinical setting.

Keywords::

• adenovirus
• genetic modifications
• gene therapy
• polymer-coating
• transductional targeting