Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization

Abstract

Introduction: Despite tremendous advances in the application of biophysical methods in drug discovery, the preponderance of instruments and techniques still require sophisticated analyses by dedicated personnel and/or large amounts of frequently hard-to-produce proteins. A technique which carries the promise of simplicity and relatively low protein consumption is the differential scanning fluorometry (DSF). This technique monitors protein through the use of environmentally sensitive fluorescent dye, in a temperature-ramp regime by observing the gradual exposure to the solvent of otherwise buried hydrophobic faces of protein domains.

Areas covered: This review describes recent developments in the field of DSF. This article pays a particular emphasis on the advances published during the 2010 – 2013 period.

Expert opinion: There has been a significant diversification of DSF applications beyond initial small molecule discovery into areas such as protein therapeutic development, formulation studies and various mechanistic investigations. This serves as a further indication of the broad penetration of the technique. In the small molecule arena, DSF has expanded toward sophisticated co-dependency MOA tests, demonstrating the wealth of information which the technique can provide. Importantly, the first public deposition of a large screening dataset may enable the use of thermal stabilization data in refining in silico models for small molecule binding.

Keywords::

• chemical chaperones
• differential scanning fluorometry
• fluorescence thermal shift
• inhibitor mechanism of action
• pharmacological chaperones
• thermal stabilization

Notes

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