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Abstract
Evaluation of: Albrecht C, Fechner P, Honcharenko D et al. A new assay design for clinical diagnostics based on alternative recognition elements. Biosens. Bioelectron. 25(10), 2302–2308 (2010).
This article focuses on a novel concept regarding the use of polypeptide conjugates as high-affinity binders to recognize targets in immunoassays. The polypeptide conjugate binder is a hybrid molecule in which a small recognition ligand is covalently linked to a synthetic polypeptide, which binds to a protein target of interest with high affinity and selectivity. Affinity of the polypeptide conjugate to its target can be tuned by changing the length of the aliphatic spacer in between the small recognition ligand and the polypeptide. Other pros and cons of this new approach for protein recognition and sensing are compared with other immunoassays such as ELISA, the bio-barcode assay and the aptamer-based barcode assay. It is anticipated that there will be more innovation and integration using new materials and technologies to provide novel ways to detect disease markers.
Financial & competing interests disclosure
The authors wish to thank the Hong Kong Research Grant Council for providing funding support under project number 413310. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.