Does additional hybridization also improve preimplantation genetic screening results?

Abstract

Evaluation of: Mir P, Rodrigo L, Mateu E et al. Improving FISH diagnosis for preimplantation genetic aneuploidy screening. Hum. Reprod. 25(7), 1812–1817 (2010).

Mir et al. have evaluated the impact of additional hybridization rounds on FISH accuracy in preimplantation genetic screening (PGS) of aneuploidy. In a retrospective analysis of 1000 PGS cycles, embryo biopsy was performed on day 3. A greater number of embryos were diagnosed as chromosomally normal and were available for transfer after discarding ‘false monosomies’ and decreasing the number of noninformative embryos. The error rate for the FISH technique was 17.3% without and 5% with additional probes. A randomized controlled trial is needed to demonstrate improved clinical outcome. However, major obstacles in cleavage-stage PGS are chromosomal mosaicism and incomplete analysis. Therefore, polar body and/or trophectoderm analysis using 24 chromosome microarrays might be better alternatives.

Keywords::

• FISH accuracy
• preimplantation genetic screening
• rehybridization
• subtelomeric probes

Financial & competing interests disclosure

The author has no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.