Association Between Serum Albumin Levels and Diabetic Peripheral Neuropathy Among Patients with Type 2 Diabetes: Effect Modification of Body Mass Index

Abstract

Background

The role of serum albumin in the risk of diabetic peripheral neuropathy (DPN) remains unclear. This study aimed to explore the relationship between serum albumin level and DPN, and to examine any possible effect modifiers among patients with type 2 diabetes mellitus.

Methods

This cross-sectional study was conducted in Jiangxi, China, from May 2012 to December 2014. Serum albumin levels were determined in 523 subjects, and the association between serum albumin level and DPN was evaluated using linear regression models (odds ratio [OR] and corresponding 95% confidence interval [CI]).

Results

There was a significant inverse association between serum albumin level (per 1 g/L increment, OR 0.95 [95% CI 0.92–0.98]) and the risk of DPN. Accordingly, when serum albumin was assessed as quartiles, a significantly lower risk of DPN was found in participants in quartile 4 (OR 0.49 [95% CI 0.25–0.95]), compared with those in quartile 1. Consistently, higher albumin levels (≥35 g/L) were associated with decreased odds for DPN (OR 0.36 [95% CI 0.17–0.74]) compared with lower levels. Furthermore, the albumin–DPN association was significantly stronger in patients with a relatively high body mass index (BMI; ≥24 kg/m²; OR 0.91 [95% CI 0.85–0.98]) than in those with a low BMI (<24 kg/m²; OR 0.99 [95% CI 0.94–1.04]; P for interaction = 0.042).

Conclusion

These data suggest that serum albumin level could be a novel risk factor for DPN among patients with type 2 diabetes and relatively high BMI (>24 kg/m²).

Keywords:

• serum albumin
• BMI
• diabetic peripheral neuropathy
• type 2 diabetes mellitus

Introduction

Diabetic peripheral neuropathy (DPN) is the most frequent long-term complication among patients with type 2 diabetes mellitus (T2DM), resulting in peripheral nerve dysfunction accompanied by the typical characteristics of pain, dysesthesia, paresthesia, and numbness.^1,2 It affects approximately 50% of patients over the course of disease, with high morbidity and mortality.³ Therefore, it is essential to identify and treat potential risk factors for the occurrence of DPN to improve prevention and reduce its significant disease-related burden.

Albumin is the most abundant serum protein and is exclusively synthesized in the liver and subsequently secreted into the plasma, entering both the intra- and extravascular spaces.⁴ Serum albumin has often been identified as a marker of nutritional status (malnutrition/overnutrition) and inflammation;⁵ however, its role as a potential endogenous antioxidant agent under physiological conditions is being increasingly recognized.⁶ Accordingly, previous studies have demonstrated the obvious protective influence of serum albumin in many diseases, such as myocardial infarction and cardiovascular diseases.^7–9 Some reports have also suggested that relatively low serum albumin levels in patients who experienced acute ischemic stroke increased the risk of poor outcome or death.¹⁰ In addition, we previously reported that elevated albumin levels protect against the progression of chronic kidney disease.¹¹

Potential risk factors for DPN need to be identified, despite their complexity and difficulty. DPN can be affected by many factors, such as hyperglycemia, age, hypertension, smoking status, and hyperlipidemia.^12–15 However, the cause of DPN remains to be comprehensively elucidated. Patients with diabetes, especially elderly individuals with malnutrition, often experience peripheral neuropathy.^16,17 Based on the close connection between hypoproteinemia and malnutrition,^5,18 we hypothesized that serum albumin levels could be used as an early predictor of DPN. However, to the best of our knowledge, only two studies have examined the association between serum albumin concentration and DPN among patients with T2DM.^19,20 Furthermore, none of the previous studies has comprehensively investigated modifiers in the association between serum albumin and DPN. Therefore, the objective of the present study was to evaluate the association between serum albumin levels and the prevalence of DPN among patients with T2DM. We also assessed whether this association persisted when other potential confounders were considered.

Materials and Methods

Subjects and Study Design

Subjects included in this study were patients with T2DM routinely hospitalized in the Department of Endocrinology and Metabolism at the First Affiliated Hospital of Nanchang University (Nanchang, China) between May 2012 and December 2014. Inclusion criteria were as follows: age ≥ 18 years; diagnosis of T2DM; and voluntarily participation and provision of informed written consent at admission. Patients with typical clinical features of type 1 diabetes, those with another specific form of diabetes, such as diabetes secondary to chronic pancreatitis or steroid treatment, those with acute myocardial infarction, heart failure, or cerebrovascular disease, kidney disease, such as urolithiasis, cancer, infection, acute diabetic complications, and other severe circulatory, respiratory, or digestive system diseases were excluded. T2DM was defined as patients with self-reported, physician-diagnosed diabetes, those taking anti-diabetic medications, or a fasting glucose level ≥7.0 mmol/L according to criteria from the World Health Organization.²¹ A total of 523 patients were enrolled, and hospitalization data from all subjects were retrospectively analyzed.

DPN

Diagnostic criteria for DPN included the following: among patients with clinical symptoms (pain, numbness, paresthesia), any one of five examinations (ankle reflex, acupuncture pain, vibration, pressure, and temperature) being abnormal according to the patient’s diagnosis of neuropathy during or after diabetes; in the absence of clinical symptoms, 2 of 5 abnormalities could be diagnosed with DPN; and abnormalities revealed on nerve conduction study (ApS, Alpine Biomed, Skovlunde, Denmark), which included motor nerve conduction studies (median, ulnar, tibial, and common peroneal nerves) and sensory nerve conduction studies (median, ulnar, superficial peroneal, and sural nerves). Distal motor latency, motor nerve conduction velocity, compound muscle action potential wave amplitude, distal sensory latency, sensory nerve conduction velocity, and sensory nerve action potential wave amplitude were measured.

Collection of Demographic Information, and Medical and Laboratory Data

For this hospital-based cross-sectional study, all patients underwent standardized clinical and laboratory evaluations. Basic demographic data, including age, sex, height, weight, smoking status, systolic blood pressure (SBP), and diastolic blood pressure, were collected from medical records. Weight and height were determined in subjects wearing light clothing and no shoes by an ultrasonic instrument (Omron HNH–318, Japan). Body mass index (BMI) was calculated by dividing weight in kilograms by height in meters squared (kg/m²). Blood and urine samples collected on admission were analyzed during the study period. Fasting blood samples were collected to measure fasting plasma glucose (FPG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol, triglycerides, and albumin levels and were analyzed using an automated chemistry analyzer (AU5421, Olympus, Shizuoka, Japan). Glycated hemoglobin (HbA1c) content was measured using a glycated hemoglobin analyzer (D-10, Bio-Rad, Hercules, CA, USA).

Statistical Analysis

Continuous variables were analyzed using the t-test and expressed as mean ± standard deviation (SD). For categorical variables, the chi-square test or Fisher’s exact test was used, and data are expressed as percentage. The association between serum albumin and DPN was evaluated using linear regression models (odds ratio [OR] and corresponding 95% confidence interval [CI]), with and without adjustments for age, sex, BMI, FPG, HbA1c, TC, and LDL-C. In addition, possible modifications of the association between serum albumin (per 1 g/L increment) and DPN were assessed for the following variables: age (< 60 versus [vs] ≥ 60 years), sex, BMI (< 24 vs ≥ 24 kg/m²), TC (< 5.2 vs ≥ 5.2 mmol/L), FPG (< 6.1 vs ≥ 6.1 mmol/L), and HbA1c (< 6.5% vs ≥ 6.5%). Heterogeneity across subgroups was assessed using linear regression models and presented in forest plots, and interactions between subgroups and serum albumin concentrations were examined using likelihood ratio testing.

Differences with a two-tailed P-value of <0.05 were considered to be statistically significant in all analyses. R software (http://www.R-project.org) was used for all statistical analyses.

Results

Clinical Characteristics of the Participants

A total of 523 patients with T2DM were enrolled in the present study, including 403 in the DPN group and 120 in the non-DPN group. The demographic and clinical characteristics of the DPN and non-DPN groups are summarized in Table 1. The mean age was 59.9 ± 10.7 years, and 52.4% of the patients were male. The mean concentration of serum albumin was 39.9 ± 7.2 g/L. Patients with DPN were older, and exhibited greater FPG and LDL-C, and lower albumin levels.

Table 1 Characteristics of the Study Participants Without and with Diabetic Peripheral Neuropathy*

Characteristics	Total	Without DPN	With DPN	P value*
No. of participants	523	120	403
Age, year	59.3 (10.7)	56.2 (11.0)	60.2 (10.4)	<0.001
Male, No. (%)	274 (52.4)	69 (57.5)	205 (50.9)	0.202
BMI, kg/m^2	24.1 (3.8)	24.1 (4.4)	24.1 (3.6)	0.915
Current smoker, No. (%)	67 (13.6)	13 (11.4)	54 (14.3)	0.432
Blood pressure, mmHg
Systolic BP at baseline	134.7 (19.0)	131.4 (17.7)	135.8 (19.2)	0.029
Diastolic BP at baseline	83.2 (11.5)	82.8 (10.9)	83.3 (11.7)	0.694
NRF2 polymorphisms				0.090
CC	228 (43.6)	46 (38.3)	182 (45.2)
AC	246 (47.0)	57 (47.5)	189 (46.9)
AA	49 (9.4)	17 (14.2)	32 (7.9)
Laboratory results
Fasting glucose, mmol/L	8.4 (4.3)	7.4 (3.6)	8.5 (4.4)	0.010
HbA1c (%)	8.6 (2.3)	8.3 (2.3)	8.7 (2.4)	0.091
Total cholesterol, mmol/L	4.3 (1.2)	4.2 (1.4)	4.4 (1.1)	0.083
Triglycerides, mmol/L	1.6 (1.5)	1.6 (1.7)	1.5 (1.4)	0.494
LDL-C, mmol/L	2.6 (0.9)	2.5 (0.8)	2.7 (0.9)	0.039
HDL-C, mmol/L	1.3 (4.7)	1.2 (0.5)	1.4 (5.3)	0.663
Serum albumin, g/L	39.9 (7.2)	42.1 (7.5)	39.3 (7.0)	<0.001

Note: *Continuous variables are presented as mean (standard deviation), categorical variables are presented as numbers (percentages).

Abbreviations: DPN, diabetic peripheral neuropathy; BP, blood pressure; BMI, body mass index; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.

Patients were divided according to quartiles of serum albumin levels. Higher serum albumin levels were associated with decreased FPG and HbA1c levels, and decreased levels of TC and LDL-C (Table 2).

Table 2 Characteristics of the Study Participants Stratified by Quintiles of Serum Albumin*

Characteristics	Q1	Q2	Q3	Q4	P value*
No. of participants	128	133	131	131
Age, year	59.6 (10.7)	62.6 (9.9)	58.6 (10.5)	57.0 (11.1)	<0.001
Male, No. (%)	71 (50.4)	70 (50.7)	76 (48.7)	88 (60.3)	0.181
BMI, kg/m^2	23.9 (3.1)	23.5 (4.8)	24.7 (4.1)	24.6 (3.7)	0.134
Current smoker, No. (%)	23 (17.4)	11 (8.1)	16 (11.0)	12 (16.1)	0.081
NRF2 polymorphisms
CC	66 (46.8)	51 (37.0)	71 (45.5)	66 (45.2)	0.560
AC	63 (44.7)	76 (55.1)	69 (44.2)	66 (45.2)
AA	12 (8.5)	11 (8.0)	16 (10.3)	14 (9.6)
Blood pressure, mmHg
Systolic BP at baseline	134.6 (22.2)	135.2 (19.1)	134.6 (18.9)	135.7 (15.9)	0.962
Diastolic BP at baseline	81.2 (13.9)	82.9 (11.0)	82.6 (10.7)	85.1 (11.8)	0.052
Laboratory results
Fasting glucose, mmol/L	9.6 (5.9)	8.5 (3.5)	8.0 (4.0)	7.4 (3.2)	<0.001
HbA1c (%)	9.5 (3.0)	8.8 (2.3)	8.5 (2.3)	7.9 (1.8)	<0.001
Total cholesterol, mmol/L	3.9 (1.1)	4.2 (0.9)	4.5 (1.4)	4.7 (1.4)	<0.001
Triglycerides, mmol/L	1.3 (0.8)	1.4 (1.2)	2.2 (4.1)	1.6 (1.2)	0.009
LDL-C, mmol/L	2.4 (1.0)	2.5 (0.8)	2.5 (0.9)	2.8 (1.0)	<0.001
HDL-C, mmol/L	1.0 (0.4)	1.1 (0.4)	1.2 (0.4)	1.9 (8.8)	0.298
Serum albumin, g/L	31.2 (6.2)	38.3 (1.0)	41.6 (1.0)	47.3 (5.8)	<0.001

Note: *Continuous variables are presented as mean (standard deviation), categorical variables are presented as numbers (percentages).

Abbreviations: BP, blood pressure; BMI, body mass index; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.

Association Between Serum Albumin Level and DPN

There was a significant inverse association between serum albumin level and the prevalence of DPN (Figure 1). Each 1 g/L increase in albumin level was associated with a 5% (OR 0.95 [95% CI 0.92–0.98]) reduction in the adjusted risk of DPN. Consistently, when albumin was assessed as quartiles, the multivariate-adjusted ORs for participants in the highest quartile (ie, Q4, ≥ 43.5 g/L) of serum albumin were 0.49 (95% CI 0.25–0.95) compared with those in quartile 1. When albumin was assessed as a categorical variable based on clinical cut-off values, compared with patients with low albumin concentrations (<35 g/L), those with normal albumin concentrations (≥ 35 g/L) exhibited lower odds of DPN (OR 0.36 [95% CI 0.17–0.74]) (Table 3).

Table 3 Association of Serum Albumin Levels and Diabetic Peripheral Neuropathy

Albumin, g/L	No.	OR (95% CI)
		Unadjusted	Adjusted	P value*
Continuous	523	0.94 (0.91, 0.97)	0.95 (0.92, 0.98)	<0.001
Quartiles
Q1 (<36.9)	128	1.00	1.00
Q2 (36.9–40.4)	133	0.65 (0.34, 1.24)	0.61 (0.31, 1.17)	0.137
Q3 (40.4–43.5)	131	0.48 (0.26, 0.89)	0.53 (0.28, 1.00)	0.051
Q4 (≥43.5)	131	0.43 (0.23, 0.79)	0.49 (0.25, 0.95)	0.035
P for trend		0.004	0.036
Clinical cutoff
<35	87	1.00	1.00
≥35	436	0.34 (0.16, 0.70)	0.36 (0.17, 0.74)	0.006

Note: *Adjusted for age, sex, body mass index, fasting plasma glucose, glycated hemoglobin, total cholesterol, as well as low-density lipoprotein cholesterol.

Figure 1 Association between serum albumin levels with diabetic peripheral neuropathy in patients with type 2 diabetes mellitus*.

Note: *Adjusted for age, sex, body mass index, fasting plasma glucose, glycated hemoglobin, total cholesterol, as well as low-density lipoprotein cholesterol.

Stratified Analysis According to Potential Effect Modifiers

Stratified analyses were performed to further assess the association between serum albumin levels and DPN among the various subgroups. A significant interaction was observed between albumin level and BMI and DPN (P = 0.042). Among patients with a high BMI (≥24 kg/m²), higher albumin concentrations were significantly associated with a lower risk of DPN (per 1 g/L increase, OR 0.91 [95% CI 0.85–0.98]). Among patients with low BMI (< 24 kg/m²), however, higher albumin concentrations had no significant effect on DPN (OR 0.99 [95% CI 0.94–1.04]) (Figure 2).

Figure 2 Association between serum albumin and diabetic peripheral neuropathy in various subgroups*.

Note: *Adjusted for age, sex, body mass index, fasting plasma glucose, glycated hemoglobin, total cholesterol, as well as low-density lipoprotein cholesterol, if not stratified.

None of the other variables, including age, sex, TC, FPG, or HbA1c, significantly modified the association between albumin and DPN (all P for interaction > 0.05).

Discussion

In this study, we investigated the relationship between serum albumin level and DPN among patients with T2DM. We found an independent inverse association between serum albumin levels and the prevalence of DPN, even after adjustment for multiple confounding factors responsible for the pathogenesis of DPN. Moreover, our study further extends the results of previous related studies by demonstrating that the association between albumin and DPN can be modified by BMI.

Only a few studies have examined the association between serum albumin level and DPN. A previous study showed that the association between serum albumin and peripheral nerve function was significant only in patients with T2DM and albuminuria.¹⁹ Additionally, in a Japanese study involving 130 patients with T2DM,²⁰ Iwasaki et al reported that serum albumin level was significantly associated with the severity of neuropathy. In the current study, we found that the association between albumin level and DPN could be modified by BMI in patients with T2DM. As such, the present study provides novel evidence supporting the neuroprotective effects of serum albumin and has shown for the first time that albumin improves DPN in patients with high BMI (≥24 kg/m²).

DPN has been associated with the effects of chronic hyperglycemia on all peripheral nerves.²² Long-term exposure to hyperglycemia causes oxidative stress, which has been reported to be associated with microvascular complications in T2DM.^23–25 There is accumulating evidence supporting the concept that oxidative stress is a biochemical trigger for nerve dysfunction and improved sensory nerve conduction velocity in diabetic rats.^26,27 Notably, the generation of reactive oxygen species and subsequent mitochondrial and DNA damage in diabetic neuropathy cause microangiopathy, ischemia, and, ultimately, peripheral nerve malfunction.²⁸ Accordingly, the treatment of diabetic rats with insulin or antioxidants is associated with improved nerve function.²⁹ Therefore, the potential neuroprotective role of albumin may be related to its antioxidant properties.

Serum albumin level is widely used as an index of nutritional status.⁵ BMI is another important variable for assessing nutritional status.³⁰ Therefore, it is possible that the combination of BMI and serum albumin level reflects the state of nutritional deficiency in greater detail. A prospective cohort study found that a combination of low BMI and serum albumin levels in patients with chronic kidney disease (stages 2–5) predicts a rapid decline in renal function more accurately than either factor alone.³¹ In addition, poor functional recovery from stroke has been noted in a population of patients with low BMI and serum albumin levels.³² These results suggest that the combination of BMI and serum albumin level can be a more robust nutritional marker. Several previous studies have investigated the relationship between BMI and DPN.^33,34 Accordingly, in the current study, there was a significant inverse association between serum albumin level and the prevalence of DPN among patients with a high BMI. However, the exact mechanism(s) by which serum albumin improves DPN in patients with high BMI remains to be further investigated, and our findings and hypotheses warrant further confirmation.

The strengths of this study include the strict enrolment criteria and the ability to perform detailed, careful assessment of patients with T2DM. All staff performing vascular and foot assessments, data collection, and entry were carefully trained and accredited to ensure data quality. Second, we used subgroup analysis to identify the interaction between serum albumin level and BMI. To the best of our knowledge, this is the first study to demonstrate the effect of BMI on the association between serum albumin and the prevalence of DPN. Finally, we adjusted for as many confounding factors as possible. These considerations suggest that the results were reliable.

Nevertheless, our study has some limitations that should be considered when interpreting the results. First, the present investigation was cross-sectional in design, which limited our ability to infer a causal association between serum albumin levels and the risk of developing DPN. Second, the patients were admitted to university hospitals; therefore, our study results may not be applicable to the general population or patients with T2DM in primary care settings. As such, further prospective studies with larger sample sizes are required to confirm the findings of this study.

In general, we devoted little attention to low serum albumin concentration as a risk factor for DPN, although serum albumin concentration can be measured easily in the clinical laboratory and used in medical practice. Therefore, it is important to clarify the clinical significance of serum albumin levels in DPN and stratify high-risk groups for DPN according to serum albumin levels. More importantly, our findings revealed the potential of albumin as an interventional target for DPN.

Conclusions

In conclusion, our data suggest that serum albumin level was independently associated with DPN among patients with T2DM, especially among those with a high BMI (≥ 24 kg/m²). Further studies are needed to verify whether modulating albumin levels could be beneficial to patients with DPN.

Ethics Approval and Consent to Participate

This study was approved by the ethics committee of the First Affiliated Hospital of Nanchang University (no. CDYFY2014009) and written informed consent was obtained from all individuals. This study was performed in adherence to the principles of the Declaration of Helsinki.

Disclosure

All authors declare they do not have conflicts of interest.

Acknowledgments

All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; agreed to submit to the current journal; gave final approval of the version to be published; and agree to be accountable for all aspects of the work.

Additional information

Funding

The study was supported by funding from the following: the National Natural Science Funds of China (Nos. 81760168 and 81460018) and the National Natural Science Funds of Jiangxi Province (No. 20192BAB205031).