High Serum Uric Acid Was a Risk Factor for Incident Asthma: An Open Cohort Study

Abstract

Background

Several cross-sectional studies have suggested an association between SUA and asthma. However, few studies have investigated this relationship longitudinally. Although SUA is an independent risk factor for chronic kidney disease and cardiovascular diseases, its contribution to incident asthma remains uncertain.

Objective

To determine whether a high SUA was a risk factor for adult incident asthma.

Methods

By using health care data from the Shandong multicenter health check-up, 76,369 participants, aged 20 to 79 years, were identified who had an SUA determination with a mean follow-up period of 3.73±2.21 years. Multivariate modeling employed Cox proportional hazards models to verify the association between SUA and incident asthma by adjusting age, BMI, smoking habits, drinking habits, and asthma-like diseases.

Results

A total of 51,389 individuals were included in the analysis. Cut-off values of SUA able to discriminate asthma status were identified by means of maximally selected rank statistics in the whole participants (≥376.80umol/L), women (≥314.45umol/L), and men (≥376.80umol/L). Multivariate Cox regression analyses adjusted for covariates (age, body mass index (BMI), smoking habit, drinking habit, and asthma-like disease) identified an independent association between SUA and incident asthma in the whole participants (hazard ratio (HR) 2.92, 95% confidence intervals (CI), 1.76–4.48, P=0.00) and men (HR 3.02, 95% CI 1.70–5.39, P=0.00), but not in women.

Conclusion

The results of the current study confirmed that high SUA was an independent risk factor for incident asthma after adjustment of potential covariates and suggested that a cut-off value related to incident asthma could be identified only in men.

Keywords:

• cut-off value
• asthma
• gender
• serum uric acid
• risk factor

Asthma is one of the most common non-communicable and chronic respiratory diseases in children and adults, and affects approximately 334 million people worldwide.¹ It has a significant impact on those affected, particularly when taking a patient’s quality of life, absenteeism, and substantial economic burden into account.^2–4 However, there is no treatment available to cure asthma.⁵ Therefore, it is particularly important to find the risk factors of asthma to prevent the occurrence of asthma.

Uric acid (UA) is the final product of nucleic acid metabolism and is the most abundant molecule with antioxidant properties found in human blood serum.^6,7 Serum uric acid (SUA) is measured frequently in the primary care setting to assess renal function, diagnose gout, and monitor drug response.^8,9 Recently, SUA has been suggested to have an association with hypertension, metabolic syndrome, and cardiovascular diseases.^10–12 However, the relationship between SUA and respiratory disorders remained unclear. Studies have shown that UA was a known trigger for acute neutrophilic inflammation and an inflammatory mediator of allergic asthma.^13,14 Besides, SUA levels increased at the onset of asthma exacerbation, and had a negative impact on spirometric pulmonary functions in asthmatics.^15,16 However, at present, whether the level of SUA affects the occurrence of incident asthma is uncertain.

Some studies supported an independent association between both SUA and hypertension, as well as cardiovascular disease, and it was also clear that these associations were stronger in women.¹⁷ However, whether there are gender differences in the relationship between SUA and asthma has not been investigated by researchers.

For the specific purpose of the analysis shown herein, we conducted an open cohort study to explore the relationship between SUA and incident asthma both men and women. We hypothesized that a higher SUA was associated with a higher risk of incident asthma.

Methods

Study Design

The study was an open prospective cohort study that examined the relationship between SUA at baseline and incident asthma with a mean follow-up period of 3.73±2.21 years. The study used health care data from over 20 health management centers in Shandong Province from January 2004 to December 2015 to identify participants with incident asthma, and the study design is shown in Figure 1. The study was approved by the Institutional Ethical Committee, School of Nursing, Cheeloo College of Medicine, Shandong University (2003-R-036). The study was in compliance with the Declaration of Helsinki for clinical research. All participants provided written informed consent before participating in the study.

Figure 1 Study design.

The database was built on a regional basis by collecting data on individuals from representative cohorts having SUA measurement and complete information about several variables. For all individuals, a standardized set of items was recorded, including demographics, smoking habits, drinking habits, height, weight, and asthma-like diseases (rhinitis, nasal polyps, pneumonia, bronchitis, pulmonary infection, bronchiectasis, and chronic obstructive pulmonary disease (COPD)).

Inclusion and Exclusion Criteria

The inclusion criteria were as follows: (1) aged ≥20 years; (2) had regular physical examination (once a year) over three times during follow-up; (3) free from asthma at baseline; and (4) individuals who provided informed consent to participate voluntarily; Exclusion criteria: participants had an undetermined baseline SUA.

Diagnostic Criteria for Asthma

The diagnostic criteria for asthma were according to the guidelines for Asthma Section, Respiratory Disease Branch, Chinese Medical Association.¹⁸

Statistical Analysis

The R package version 4.0.2 and IBM SPSS Statistics 24 were used for statistical analysis.

General Description

Continuous variables were reported as mean ± standard deviation or median (interquartile range [IQR]), and categorical variables as frequency (percentage). Between-group comparisons were performed using the t-test or Wilcoxon rank-sum test for continuous variables and using the chi-square test for categorical variables.

Preliminary Cox Analysis

SUA as a continuous item was used as the independent variable in Cox analyses both in the whole participants and separately in men and women, and age, BMI, smoking habits, drinking habits, and asthma-like disease as possible covariates. HR with 95% CI were produced. The null hypothesis was rejected for values of P less than 0.05.

Univariate Cut-off Values

We estimated the optimal cut-off value of SUA for predicting the occurrence of incident asthma using maximally selected rank statistics.¹⁹ This cut-off value was optimal for separation into high and low levels of SUA. Statistically significant differences using this cut‐off value were examined using P values.

Kaplan–Meier Curves

In the whole participants and separately in women and men, the cumulative risk of SUA cut-off points and asthma incidence over time was calculated using the Kaplan–Meier cumulative hazard function, and Log rank tests were used to evaluate the differences between groups.

Validation of the Cut-off Values

The cut-off values of SUA identified by means of the maximally selected rank statistics were tested in two ways: they were used separately as independent variables in multivariate Cox analyses adjusted for the covariates, the occurrence of incident asthma as the dependent variable. The analysis was performed on the whole participants as well as in men and women. The corresponding HR and 95% CI were obtained; Besides, considering the low incidence of asthma in this study, we also performed the Poisson regression model to verify the reliability of the results.

Results

Descriptive Statistics

Among the 76,368 original participants at the baseline examination in this study, 51,389 eligible subjects were included finally. In total, 88 eligible subjects developed incident asthma during the follow-up, including 36 women (40.91%) and 52 men (59.09%) (Figure 2).

Figure 2 Overview of study participant.

General characteristics of the 51,389 individuals are shown and also stratified by gender in Table 1. During 176,643 person-years of follow-up, 88 participants experienced incident asthma: 52 events occurred in men during 108,175 person-years of follow-up and the crude incidence density of asthma was 0.48 per 1000 person-years; 36 events occurred in women during 68,468 person-years of follow-up, and the crude incidence density of asthma was 0.53 per 1000 person-years.

Table 1 Characteristics of 51,389 Participants Included in the Study

Characteristics	Men (30,847)			Women (20,542)			Total Participants (n=51,389)
	Asthma (n=52)	Control (n=30,795)	P	Asthma (n=36)	Control (n=20,506)	P	Asthma (n=88)	Control (n=51,301)	P
Age, years, mean ± SD	47.25±11.68	40.73±12.61	0.00*	47.89±12.52	41.54±13.39	0.00*	47.51±11.97	41.05±12.94	0.00*
Age, years, no.(%)			0.00*			0.00*			0.00*
20–30	2(3.85)	7706 (25.02)		3(8.33)	5275(25.72)		5 (5.68)	12,986 (25.31)
30–40	17 (32.69)	8764 (28.41)		6(16.67)	5150(25.11)		23 (26.14)	13,937(27.17)
40–50	13 (25.00)	7862 (25.53)		14(38.89)	5026(24.51)		27 (30.68)	12,915(25.17)
50–60	12 (23.08)	3952 (12.83)		7(19.44)	2997(14.62)		19 (21.59)	6968(13.58)
>60	8 (15.38)	2511 (8.15)		6(16.67)	2058(10.04)		14 (15.91)	4583(8.93)
BMI, kg/m^2, mean ± SD	25.35±3.50	25.67±15.46	0.90	24.60±3.01	23.68±5.09	0.09	25.04±3.31	24.88±12.44	0.90
BMI, kg/m^2, no.(%)			0.82			0.12			0.50
<18.5	1 (1.92)	430 (1.40)		0(0.00)	1205(5.88)		1(1.14)	1636(3.19)
18.5–23.9	20 (38.46)	8781 (28.51)		14(38.89)	9436(46.02)		34(38.64)	18,251(35.58)
24.0–27.9	20 (38.46)	14,855 (48.24)		15(41.67)	7839(39.23)		35(39.77)	22,729(44.31)
>28.0	11 (21.15)	6729 (21.85)		7(19.44)	2026(9.88)		18(20.45)	8773(17.10)
Drinking status	21 (40.38)	7011 (22.77)	0.01*	0(0.00)	42(0.20)	/	21(23.86)	7053(13.75)	0.01*
Smoking status	17 (32.69)	5667 (18.40)	0.00*	0(0.00)	32 (0.16)	/	17(19.32)	5699(11.11)	0.01*
Respiratory diseases
Rhinitis	1 (1.92)	101 (0.33)	0.20	0(0.00)	32 (0.16)	/	1(1.14)	133 (0.26)	0.11
Nasal polyps	2 (1.92)	44 (0.14)	0.00*	0(0.00)	13(0.06)	/	2 (2.27)	57 (0.11)	0.00*
Pulmonary infection	1 (1.92)	122 (0.40)	0.11	1(5.26)	92(0.45)	0.04*	2 (2.27)	214 (0.42)	0.01*
Pneumonia	8 (13.46)	294 (0.95)	0.00*	7(18.42)	210(1.02)	0.00*	15 (17.05)	504 (0.98)	0.00*
Bronchitis	8 (15.38)	320 (1.04)	0.00*	10(26.32)	253(1.23)	0.00*	18 (20.45)	573 (1.12)	0.00*
COPD	6 (13.46)	75 (0.24)	0.00*	4(7.89)	20(0.10)	0.00*	10 (11.36)	95 (0.19)	0.00*
Bronchiectasis	1 (1.92)	37(0.12)	0.00*	0(0.00)	22(0.11)	/	1(1.14)	59 (0.12)	0.01*

Note: *P<0.05/Invalid data.

Abbreviations: BMI, body mass index; COPD, chronic obstructive pulmonary disease.

Multivariate Analysis

Preliminary Cox models having incident asthma as a dependent variable showed that SUA as a continuous variable was associated with incident asthma in the whole participants and men (HR 1.00, 95% CI 1.00–1.01, P=0.01) (HR 1.01, 95% CI 1.00–1.01 P=0.00); however, SUA as a continuous item was rejected in women from the model (HR 1.00, 95% CI 0.99–1.01 P=0.56). This result was consistent with the result that SUA was used as a continuous variable without adjusting for covariates (Men: HR 1.01, 95% CI 1.00–1.01 P=0.00; Women: HR 1.00, 95% CI 0.99–1.01 P=0.09; Total: HR 1.00, 95% CI 1.00–1.01 P=0. 02) (Table 2). We also used the Poisson regression model for analysis, and the analysis results were similar to the Cox proportional hazard model analysis (Supplementary Table 1).

Table 2 Cox Models for Incident Asthma Using Serum Uric Acid as a Continuous Independent Variable

Independent Variables	Men				Women				Total
	HR	95% CI		P	HR	95% CI		P	HR	95% CI		P
Unadjusted covariates
SUA	1.01	1.00	1.01	0.00*	1.00	0.99	1.01	0.09	1.00	1.00	1.01	0.02*
Adjusted covariates
SUA	1.01	1.00	1.01	0.00*	1.00	0.99	1.01	0.56	1.00	1.00	1.01	0.01*
Age	1.03	1.01	1.06	0.01*	1.03	1.01	1.06	0.02*	1.03	1.01	1.05	0.00*
BMI	0.99	0.91	1.08	0.83	1.02	0.98	1.05	0.36	1.00	0.99	1.01	0.72
Gender	/	/	/	/	/	/	/	/	2.27	1.30	3.97	0.00*
Drinking status	1.13	0.62	2.06	0.69	/	/	/	/	0.88	0.51	1.51	0.65
Smoking status	1.30	0.71	2.40	0.40	/	/	/	/	1.05	0.59	1.85	0.87
Rhinitis	1.77	0.17	18.77	0.64	/	/	/	/	1.32	0.13	13.16	0.81
Nasal polyps	7.59	1.33	43.31	0.02*	/	/	/	/	8.10	1.54	42.67	0.01*
Pulmonary infection	0.22	0.02	1.95	0.17	0.82	0.10	6.85	0.85	0.31	0.07	1.42	0.13
Pneumonia	5.05	2.02	12.62	0.00*	2.79	0.98	7.95	0.05*	4.21	2.10	8.45	0.00*
Bronchitis	3.81	1.46	9.93	0.01*	11.14	4.67	26.56	0.00*	5.96	3.09	11.48	0.00*
COPD	9.21	2.90	29.31	0.00*	14.39	3.97	52.09	0.00*	8.83	3.74	20.85	0.00*
Bronchiectasis	1.88	0.21	16.97	0.57	/	/	/	/	1.29	0.16	10.25	0.81

Note: *P<0.05/Invalid data.

Abbreviations: BMI, body mass index; COPD, chronic obstructive pulmonary disease; SUA, serum uric acid.

Search for Cut-off Values

Maximally selected rank statistics furnished plausible univariate cut-off value of SUA for incident asthma among men, women, and whole participants, and the corresponding P value of less than 0.02 (Men: 376.80umol/L; Women: 314.45umol/L; Total: 376.80umol/L). The result is shown in Figure 3.

Figure 3 Evaluating the cutoff point of SUA level with maximally selected rank statistics (P<0.02).

Confirmation of the Cut-off Value

In multivariate Cox analyses adjusted for age, gender, BMI, smoking habits, drinking habits, and asthma-like diseases, the cut-off value of SUA for incident asthma was accepted in the model in men and all participants. (Men: HR 3.02, CI 1.70–5.39, P=0.00; Total: HR 2.92, CI 1.76–4.84, P=0.00), however, the cut-off value of SUA was rejected in the women (HR 1.75, 95% CI 0.78–3.96 P=0.18) (Table 3). We also used the Poisson regression model for analysis, and the analysis results were similar in the Cox proportional hazard model analysis (Supplementary Table 2).

Table 3 Cox Models for Incident Asthma Using Serum Uric Acid as a Binary Independent Variable

Independent Variables	Men				Women				Total
	HR	95% CI		P	HR	95% CI		P	HR	95% CI		P
Unadjusted covariates
SUA	2.53	1.46	4.37	0.00*	2.26	1.03	4.97	0.04*	2.01	1.30	3.11	0.00*
Adjusted covariates
SUA	3.02	1.70	5.39	0.00*	1.75	0.78	3.96	0.18	2.92	1.76	4.84	0.00*
Age	1.03	1.01	1.06	0.01*	1.03	1.00	1.06	0.02*	1.03	1.01	1.05	0.00*
BMI	1.00	0.92	1.08	0.91	1.02	0.98	1.05	0.35	1.00	0.99	1.01	0.66
Gender	/	/	/	/	/	/	/	/	2.11	1.22	3.64	0.01*
Drinking status	1.14	0.63	2.08	0.66	/	/	/	/	1.09	0.61	1.95	0.78
Smoking status	1.31	0.71	2.41	0.39	/	/	/	/	1.28	0.70	2.35	0.42
Rhinitis	1.44	0.13	15.65	0.76	/	/	/	/	1.24	0.12	12.73	0.85
Nasal polyps	8.48	1.48	48.63	0.02*	/	/	/	/	9.85	1.84	52.80	0.01*
Pulmonary infection	0.16	0.02	1.54	0.11	0.85	0.11	6.95	0.88	0.22	0.05	1.10	0.07
Pneumonia	5.33	2.12	13.38	0.00*	3.02	1.08	8.43	0.03*	4.31	2.16	8.62	0.00*
Bronchitis	4.08	1.56	10.65	0.00*	11.15	4.72	26.34	0.00*	6.29	3.28	12.09	0.00*
COPD	8.45	2.67	26.71	0.00*	14.10	3.99	49.86	0.00*	9.83	4.17	23.19	0.00*
Bronchiectasis	2.43	0.28	20.90	0.42	/	/	/	/	1.49	0.19	11.66	0.70

Note: *P<0.05/Invalid data

Abbreviations: BMI, body mass index; COPD, chronic obstructive pulmonary disease; SUA, serum uric acid.

Application of the Confirmed Cut-off Values

Kaplan–Meier curves are shown in Figure 4. The curves of individuals having SUA cutoff or less and SUA more than cut-off were separate without adjusting for covariates in men, women, and all participants (P<0.05).

Figure 4 Cumulative hazard function of SUA cutoff points for asthma incidence in men, women and all participants. These risk curves did not adjusted covariates. Redline (upper line) represents SUA ≥ cut-off values, and blue line (lower line) represents SUA < cut-off values. (A)The univariate cut-off value of SUA for incident asthma among men was 376.80umol/L; (B) The univariate cut-off value of SUA for incident asthma among women was 314.45umol/L; (C) The univariate cut-off value of SUA for incident asthma among total participants was 376.80umol/L.

Discussions

In the open cohort study of 51,389 individuals, we found that high SUA was an independent risk factor for incident asthma after adjusting for potential covariates, and also identified the theoretical optimal cut-off of SUA level for incident asthma. (≥376.80umol/L)

Concerning gender, the literature showed that a difference exists between women and men for SUA.²⁰ Interestingly, we divided all participants according to gender, and SUA was 28.20% lower in women than in men. We generally obtained cut-off values from a population including both genders that be of no help. Because a patient can only be male or female in clinical practice. This is why we think it is necessary to find the cut-off value for men and women separately.

The cut-off value of SUA in men identifying those at higher risk of developing incident asthma was over 376.8umol/L during the follow-up, which commonly was lower than the SUA values related to hyperuricemia. However, in women, a theoretical univariate cut-off of SUA could be identified by the maximally selected rank statistics method, but it was without no independent value in the multivariate Cox proportional hazards model and the Poisson regression model. The cut-off values identified were therefore because of men only in the whole participants.

Several studies have reported a stronger correlation between SUA and asthma. In a retrospective study performed on 217 asthma exacerbation patients, SUA levels increased at the onset of asthma exacerbation and had a negative impact on lung function.¹⁶ In a large population-based epidemiological study, Fukuhara et al reported that elevated SUA levels may predict the development of airflow limitation risk in COPD and asthma patients.²¹ The result of Abdulnaby et al showed that high SUA had a significant positive correlation with severe asthma and a negative association with spirometric pulmonary functions in hospitalized adolescents and adults with acute asthma.¹⁵ Besides, Aida et al ’s study demonstrated that there were significant inverse correlations between spirometric parameters and SUA levels in adults who participated in an annual health check.²² However, other studies have reported conflicting results. A study involving 50 adult Nigerian asthmatic patients, aged 40 to 50 years, demonstrated that the levels of UA were lower in asthmatics than in controls (P < 0.00).²³ Furthermore, a case-controlled prospective study conducted on 219 children aged 1–12 years showed that asthmatic children during exacerbation of their asthma have significantly lower serum levels of UA (p < 0.001).²⁴

There were several possible pathophysiological mechanisms that explained the relationship between SUA and incident asthma. The oxidant/antioxidant imbalance plays a pivotal role in the lung. UA, an endogenous antioxidant, is highly present in lung tissue. Pulmonary hypoxia resulted in greater purine catabolism leading to an increase in the production of UA, which indicated UA may be a useful marker of lung injury.²⁵ In addition, the airway levels of SUA are increased in patients with asthma after allergen exposure during disease exacerbation. UA in respiratory mucosa may play a central role as a sensor of allergen exposure, and may initiate and exacerbate Th2-type immunity in the airways.²⁶ In addition, higher blood UA is a protective factor against the pathological decline of lung function in female mice, because of UA significantly suppressed hydrogen peroxide,²⁷ which may explain that we have not found that UA is a risk factor for incident asthma in the female population. However, the reason why plausible multivariate cut-off values could be found in men only is not clear. The specific mechanism still needs more biochemical and clinical studies to explore in this respect.

There were some differences in the relationship between covariates and asthma in the Poisson regression model and the Cox proportional hazards model. Combining the results of the two models, we found that gender, nasal polyps, pneumonia, bronchitis, and COPD were associated with an increased risk of asthma in the general population, which was consistent with previous research results. A large cross-sectional study of 164,215 participants in Mainland China found that bronchitis, gender, COPD, and allergic pneumonia were risk factors for asthma.²⁸ In addition, patients with nasal polyps were more likely to report asthma,²⁹ which reflects the substantial interaction between the lower and upper airways and in particular between the underlying aetiological mechanisms of airways pathology.

The strength of this study shown herein is that, to our knowledge, it is the first aimed at finding the cut-off value of SUA for incident asthma in a large population. The limitations are represented by the fact that the measurement of SUA overtime was not included in this study, as some subjects did not participate in a regular checkup; The findings may not be generalizable to all healthy people and only apply for the population at risk. “population at risk” is defined as a population with a high risk of incident asthma, such as people with more asthma-like diseases (nasal polyps, bronchitis, pneumonia, or COPD)²⁸,^30–32 and an older population³³ in this study. Compared with the individuals excluded from this study because of missing SUA, the participants included in our study may have a higher risk of incident asthma. Finally, the genetic context of the association between SUA and asthma will be considered in further research.³⁴

In summary, this open cohort study provides evidence that the measurement of the SUA level might provide significant information about incident asthma in men in addition to the evaluation of traditional risk factors in clinical practice. Furthermore, high levels of SUA may become a relevant biologic marker of asthma severity in men.

Abbreviations

HR, hazard ratio; CI, confidence interval; BMI, body mass index; COPD, chronic obstructive pulmonary disease; SUA, serum uric acid; UA, uric acid; IQR, interquartile range.

Author Contributions

All authors contributed to data analysis, drafting or revising the article, have agreed on the journal to which the article will be submitted, gave final approval of the version to be published, and agree to be accountable for all aspects of the work.

Disclosure

The authors have declared no conflicts of interest. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Additional information

Funding

This work was supported by the Key Research and Development Program of Shandong Province (Grant No. 2019GSF108198), and the Humanities and Social Sciences Youth Team Project of Shandong University (Grant Nos. IFYT1811, IFYT18036, and IFYT18037). The funding bodies were not involved in the design of the study, the collection, analysis, and interpretation of data and the preparation of the manuscript.