Composite-induced toxicity in human gingival and pulp fibroblast cells

Abstract

Objective. The most important requirement for a material to be used in medical applications is its biocompatibility. Dental composite materials come into direct contact with oral tissues, especially gingival and pulpal cells. This study was performed to evaluate possible DNA damage in cells of human origin exposed to dental composites in vitro using a cytogenetic assay. Materials and methods. Two composite resins (Vertise Flow, Kalore) were tested on human gingival and pulp fibroblasts using the acridine orange/ethidium bromide viability staining and alkaline comet assay. Cultures were treated with polymerized composites in two different concentrations (20 mg/ml, 40 mg/ml) for 14 days. Chi-square and Kruskall-Wallis non-parametric test were used for the statistical analysis (p < 0.05). Results. Significant cytotoxicity was observed for 40 mg/ml of Vertise Flow in both cultures, while Kalore (40 mg/ml) showed cytotoxic effect only on human pulp fibroblasts. A significant level of DNA damage was detected for both materials and concentrations, in both cell cultures. Conclusion. If the two cell cultures are compared, the pulp cells were more sensitive to the cyto/genotoxic effects of dental composites. Based on the results, one can conclude that the use of tested materials may cause cellular damage in gingival and pulp fibroblasts in vitro.

Key Words::

• cytotoxicity
• comet assay
• composite resins
• fibroblasts
• genotoxicity test

Acknowledgements

This investigation was supported by the Croatian Science Foundation, Croatian Ministry of Science, Education and Sports as a part of Themes no. 022-0222148-2137, 065-0352851-0410 and 065- 0650444-0418.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.