Characterization of the bone matrix and its contribution to tooth loss in human cadaveric mandibles

Abstract

Objective. It is uncertain as to what extent the major bone matrix constituents, mineral and collagen, show inter-individual variation and dependence on age and sex in jawbones. The purpose of this study was to clarify this uncertainty using cadaveric mandibles and investigate the association of bone matrix with the number of existing teeth. Materials and methods. Cortical bone samples (1 × 1 cm) collected from the mental of 48 cadaveric mandibles (27 men and 21 women; age range = 56–93 years and 63–103 years, respectively) were used to quantify three bone matrix indices: mineral content, collagen content and extent of lysine hydroxylation of collagen. Associations with age and comparisons by sex were evaluated based on bone matrix indices and the numbers of existing teeth. The numbers of existing teeth were compared between the groups showing low and high bone matrix index values. Results. A great amount of inter-individual variation was seen in all bone matrix indices. No bone matrix indices were associated with age, while the number of existing teeth was negatively associated with age. The bone matrix indices and number of existing teeth did not differ by sex. The number of existing teeth was nearly twice as high in the group showing high collagen content as in the low collagen group; however, an analysis of covariance showed a significant inter-group difference not from bone matrix indices, but rather from age. Interestingly, in comparison to femoral collagen, mandibular collagen showed lower lysine hydroxylation, which can represent an aspect of bone quality. Conclusions. Mandibular bone matrix shows great inter-individual variation and is independent of age and sex, but did not show as strong a relationship with tooth loss as age. Even so, mandibular collagen may represent a unique characteristic of bone matrix and deserves to be further investigated.

Key Words::

• age
• collagen
• lysine hydroxylation
• mineral
• sex

Acknowledgments

This study was supported by Grant-in-Aid 24592945 for Scientific Research (C), Japan. The authors thank Dr Mitsuo Yamauchi, the director of the Laboratory of Collagen Biochemistry at the Dental Research Center, University of North Carolina, Chapel Hill, NC, USA, for his informative suggestions.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.