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Acta Oto-Laryngologica Volume 98, 1984 - Issue sup417
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Original Article

A Method for Measuring Polymorphonuclear Leukocyte Concentrations in Nasal Mucus

Barry Farr Department of Internal Medicine, University of Virginia School of Medicine, Charlollesville, Virginia, USA
,
Sean F. Hackett Department of Internal Medicine, University of Virginia School of Medicine, Charlollesville, Virginia, USA
,
Birgit Winther Otopalhological Laboratory, University Ear-Nose-Throat Department, Rigshospitalet, Copenhagen, Denmark
&
J. Owen Hendley Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia, USA
Pages 15-18 | Published online: 08 Jul 2009
 
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Abstract

A method is described for measuring polymorphonuclear leukocyte (PMN) concentrations in expelled nasal mucus. Nasal mucus specimens (0.05-0.15 ml) were diluted 1:60 in Hank's ba lanced salt solution. Diluted mucus was vigorously agitated to disperse contained cells, and cells were counted in a hemocytometer. The mean PMN counts in nasal mucus from healthy young adults was 7.1 × 106 PMNs/ml mucus, but large daily fluctuations were observed in PMN counts in the same individual. This method may be useful to investigate inflammatory conditions of the nasal cavity.

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