Imported brucellosis in Denmark: Molecular identification and multiple-locus variable number tandem repeat analysis (MLVA) genotyping of the bacteria

Abstract

A polymerase chain reaction was used to identify Brucella species isolated from humans in Denmark. Consecutive analysis of referred bacteria and re-examination of historical isolates identified all as Brucella melitensis. Multiple-locus variable number tandem repeat analysis (MLVA) placed the isolates in the previously defined ‘East Mediterranean’ B. melitensis group.

Keywords::

• Brucellosis
• PCR
• MLVA

Acknowledgements

Work by PLF is part of the EBLN, European Biodefence Laboratory Network (European Defence Agency EDA B-0060-ESM4-GC) coordination work on dangerous pathogens.

Declaration of interest: No conflict of interest.