Protective effects of sesame oil on 4-NQO-induced oxidative DNA damage and lipid peroxidation in rats

Abstract

Sesame oil could be considered as a potent antioxidant and dietary supplement. It possesses antimutagenic, anti-inflammatory and anti-cardiac toxicity. In the view of available findings, the current study focused on determining the protective effects of sesame oil on 4-Nitroquinoline-1-oxide (4-NQO) -induced oxidative DNA damage and lipid peroxidation (LPO) in rats. Seven groups of Wistar albino rats each with 6 either sex were used. Groups were given vehicle control and sesame oil alone orally and 4-NQO (30 mg/kg) by intraperitoneal injection. Following the four dose levels (1, 2, 4, and 8 ml/kg orally), sesame oil plus 4-NQO were also tested. After 24 hours of 4-NQO injection, blood samples were drawn by venipuncture. DNA damage (8-hydroxy-2-deoxy guanosine; 8-OHdG) and LPO were estimated. LPO from the 4-NQO-treated group was 2.5-fold higher than that of the control LPO. Pretreatment with sesame oil reduced this by 16–61%. 8-OHdG DNA damage from 4-NQO was found to be 3-fold higher than that of controls. Pretreatment with sesame oil effectively protected against DNA damage in a dose-dependent fashion. This study indicates that the antioxidant, sesame oil, effectively protected DNA damage and LPO induced by 4-NQO.

Keywords::

• Sesame oil
• 4-NQO
• LPO
• 8-OHdG

Acknowledgments

The authors would like to acknowledge Prof. Ming-Yie Liu, from the Department of Environmental and Occupational Health, National Cheng Kung University Medical College (Tainan, Taiwan), for the facilities to carry out this work successfully.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.