Corrigendum

This article refers to:

The Serotonin Transporter Expression in Drosophila melanogaster

The publisher would like to apologise on behalf of the authors. Since publication of:

The Serotonin Transporter Expression in Drosophila melanogaster

Thomas Giang¹, Steffen Rauchfuss², Maite Ogueta³ and Henrike Scholz¹

¹Department of Animal Physiology, University of Cologne, Köln, Germany

²Julius-Maximilians-University of Würzburg, Institute of Genetics and Neurobiology,Würzburg, Germany

³Departmento Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Salamanca, Spain

in the Journal of Neurogenetics, Volume 25: Pages 17–26, the authors have informed us of the following insertions and correction:

Author list:

Thomas Giang¹*, Yvonne Ritze^2,3*, Steffen Rauchfuss², Maite Ogueta^2,4 and Henrike Scholz^1,2

¹Department of Animal Physiology, University of Cologne, Köln, Germany

²Julius-Maximilians-University of Würzburg, Institute of Genetics and Neurobiology, Würzburg, Germany

³Current address: Department of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany

⁴Current address: Departmento Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Salamanca, Spain

*These authors contributed equally to the present work

Author contributions:

TG and HS developed the concept for the paper and wrote the manuscript, with the description of dSERT antibody generation and text for Figure 6 provided by YR. YR generated dSERT fusion protein as antigen and performed initial characterizations with MO of the antisera and serotonin labeling in the larval brain and in the adult brain. The data (2A) was generated by YR and Anne Haberberger which we like to thank here. TG collected data and designed figure 1, 2, 4C and 4D, 5. SR collected data for figure 3. MO developed the concept generated the data and designed figure 4A and 4B. The initial observation that there are local differences of dSERT and 5HT was made by MO. YR generated data and designed figure 6. HS generated data and designed figure 7 (was 6).

Insertion in Methods section:

Generation of antisera

A 392 base pair fragment of the dsert cDNA (RE10485) starting from the N-terminus with no homologies to other transporters from Drosophila or other species (between the nucleotide + 4586 to + 4978 relative to the transcription start side) was amplified using the linker primers AAA GAA TTC AAA TGG ACC GCA GCG GG and TTT CTC GAG CTC CGC CTT CTG TCC (EcoRI- and XhoI-linkers are underlined). The fragment was cloned directionally into the pGEX expression vector (GE Healthcare Life Science, USA). The 46 kDa GST fusion protein was purified with sepharose beads according to the manufacturer's instructions (Sigma-Aldrich, Germany) and used to immunize rabbits (Eurogenetec). The polyclonal antiserum was tested for specificity for the SERT fusion protein with western blot analysis and used at a concentration of 1:1500 – 2000 for immunohistochemistry.

Insertion in Results section after headline “dSERT Expression in Serotonergic Neurons”

The distribution of dSERT protein was also examined with the dSERT antiserum in sections of adult fly brain and co-localized with the staining of an antibody against 5-HT (Figure 6 A, B). All cell bodies that expressed dSERT were positive for 5-HT and vice versa. Examples of specific 5HT expressing cell body clusters – specifically LP1, LP2, SP1, SP2 and SE3 - that express dSERT are shown at higher magnification (Figure 6a-d’’). In addition, overlapping expression of 5-HT and dSERT was found in projections and dot-like varicosities throughout the brain (Figure 6A’’, B’’). For example partial overlap of dSERT and 5-HT expression was found in the central complex. In the ellipsoid body dSERT and 5-HT staining was co-localized in the outer ring and less pronounced in the inner or middle ring (Figure 6 A’, A’’). In the fan-shaped body, 5-HT and dSERT immunoreactive staining was mainly found in a glomerular-like pattern in the outer layers (Figure 6 A’, A’’).

Insertion of new Figure 6:

Figure 6. dSERT and 5-HT antibody staining on adult fly cryosections. dSERT staining is found widely throughout the brain in cell bodies, aborizations and varicosities (green) (A, B). The distribution of serotonergic neurons is visualized by 5-HT IR shown in magenta (A’, B’). Neurons in serotonergic LP1, LP2, SP1, SP2 and SE3 cluster show co-localization of dSERT and 5-HT antibody staining. Cluster are marked by white arrows (A’’, B’’) and are shown at a higher resolution (a-d, a’-d’, a’’-d’’). The scale bar in B” represents 50µm for A – B”, the bar in d” represents 10µm for a – d”.

Renaming of Figure 6 as Figure 7 (including all references to it).