Plasma and Aqueous Humor Angiotensin-Converting Enzyme Levels in Patients with Diabetic Retinopathy

Abstract

Purpose: To assess angiotensin-converting enzyme (ACE) levels in aqueous humor and plasma of patients with nonproliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR) as another potent ischemia-induced angiogenic factor.

Methods: The clinical comparative study included 40 patients with diabetic retinopathy and 16 healthy subjects. For all patients, aqueous humors were collected during the cataract surgery or intravitreal injection of triamcinolone acetonide. ACE levels were measured using a solid-phase chemiluminescence immunoassay.

Results: We observed significantly elevated ACE level in aqueous humor of patients with PDR compared with the patients with NPDR and normal subjects (P = 0.023), but no significant difference was detected between nonproliferative diabetics and control group (P = 0.239). There was no significant difference in plasma ACE levels among diabetic and control groups (P = 0.816).

Conclusion: Elevated ACE level may induce retinal angiogenesis and proliferative retinopathy in patients with DM. We consider that high levels of ACE in aqueous humor can reflect the association between retinal angiogenesis and DM serve as predictor in the progression of diabetic retinopathy.

KEYWORDS::

• Angiotensin-converting enzyme
• Aqueous humor
• Diabetes mellitus
• Diabetic retinopathy
• Retinal angiogenesis
• Plasma

ACKNOWLEDGMENTS

We owe special thanks to Unal Erkorkmaz, Ph.D., Department of Biostatistics for helping with statistical evaluation of the study.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and the writing of the paper.