Monocyte chemoattractant protein-1 (MCP-1) g.-2518A>G polymorphism and susceptibility to type 2 diabetes (T2D) and end stage renal disease (ESRD) in the North-West Indian population of Punjab

Abstract

Background: Diabetic nephropathy (DN) is a major microvascular complication that develops in nearly 20–30% of patients with type 2 diabetes (T2D) and is currently the leading cause of end stage renal disease (ESRD). Monocyte chemoattractant protein-1 (MCP-1), a potent chemokine secreted by adipocytes, has been implicated as a causal factor in the progression of vascular complications in T2D, thus MCP-1 appears to be a promising candidate for association study.

Aim: The objective of the present study is to evaluate the association, if any, of g.-2518A>G polymorphism (rs1024611) in MCP-1 gene in T2D cases with and without ESRD in the population of Punjab from North-West India.

Subjects and methods: A total of 571 samples from Punjab comprising 350 T2D cases (145 with ESRD and 205 without ESRD) and 221 controls were genotyped for g.-2518A>G MCP-1 polymorphism using amplification refractory mutation system- polymerase chain reaction.

Results: The frequency of G allele was observed to be higher in T2D cases with ESRD (34.49%) compared to T2D cases without ESRD (24.39%) and controls (31.67%). Under the dominant model, G allele increased the risk of ESRD by 1.68-fold [p = 0.047, OR = 1.68 (1.0–2.79) at 95% CI].

Conclusion: MCP-1 -2518 GG genotype and G allele may increase the risk of progression to ESRD in T2D cases.

• Association analysis
• ESRD
• survival analysis
• MCP-1
• T2D

Acknowledgements

The financial assistance to Priyanka Raina by DST-INSPIRE programme is acknowledged. The assistance of Dr Mohit Nagpal (Nephrologist, Fortis Escorts Hospital, Amritsar), Dr P. S. Mokha (Head, Mokha hospital, Amritsar) and Dr Virinder Singh (Head, Dr Virinder Singh kidney clinic and dialysis centre, Amritsar) in sample collection is also humbly acknowledged.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.