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Hemoglobin
international journal for hemoglobin research
Volume 39, 2015 - Issue 5
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Short Communication

Determination of Human Hemoglobin Derivatives

Atef M. M. AttiaBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt Correspondence[email protected]
,
Fatma A. A. IbrahimBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt
,
Noha A. Abd El-LatifBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt
,
Samir W. AzizBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt
,
Sherif A. Abdelmottaleb MoussaBiophysical Laboratory, Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt ;Department of Physics, College of Science, Al-Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia
&
Mohsen S. ElalfyPediatric Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Pages 371-374 | Received 04 Dec 2014, Accepted 24 Feb 2015, Published online: 21 Jul 2015
 
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Abstract

The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727–0.370%), metHb% (0.43–1.0%), HbCO% (0.4–1.52%) and oxyHb% (97.06–98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608–15.777 g/dL). The method is highly sensitive, accurate and reproducible.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

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