Effect of anionic macromolecules on intestinal permeability of furosemide

Abstract

Context: Furosemide is an anionic molecule and has very low absorption in gastro intestinal tract.

Objective: The aim of this study was to investigate the effect of anionic macromolecules on the intestinal permeability of Furosemide.

Materials and methods: The intestinal permeability of Furosemide was determined using single-pass intestinal perfusion technique in rats. Briefly a jejunal segment of ∼10 cm was isolated and cannulated in both ends for inlet and outlet solution. The perfusate was collected every 10 min and samples were analyzed using the RP-HPLC method. Test samples containing furosemide and two anionic macromolecules, sodium carboxy methyl cellulose and sodium alginate, at different concentrations were used.

Results: The obtained data showed that existence of Sodium carboxy methyl cellulose significantly increased the P_eff values in all three investigated concentrations (p < 0.05) but sodium alginate only in concentrations <0.1% increased drug permeability.

Discussion: It is concluded that the anionic macromolecules at specific concentrations could alter the permeability of anionic drugs across the biological membranes.

Conclusions: Donnan phenomenon and chelating property of macromolecules could be attributed to the observed effect.

• Donnan effect
• furosemide
• single-pass intestinal perfusion

Acknowledgements

The authors would like to thank the authorities of the Faculty of Pharmacy, Tabriz University of Medical Sciences for providing analytical facilities.

Declaration of interest

The authors report no declarations of interest. Authors thank Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran, for financial support. This paper is based on a Pharm D thesis (number 3479) submitted in Faculty of Pharmacy, Tabriz University of Medical Sciences.