In vivo and In Vitro Antitumor Effects of a Staphylococcal Enterotoxin A Mutant (SEA-H61D)

ABSTRACT

In this study, we evaluated SEA-H61D, a staphylococcal enterotoxin A mutant without emetic activity, as an antitumor agent in vitro and in vivo. It showed that SEA-H61D could significantly inhibit the growth of many cancer cell lines in vitro at very low concentrations by activating human peripheral blood mononuclear cells (PBMCs). CD4+ and CD8+ T lymphocytes could be activated at a dose between 125 and 500 μ g/kg. Systemic administration of SEA-H61D in vivo significantly inhibited tumor growth, with the treated group undergoing tumor necrosis and showing a strong infiltration of lymphocytes to the tumor area.

Keywords: :

• Superantigen
• Staphylococcal enterotoxin A
• Mutant
• Tumor model
• Immunotherapy

ACKNOWLEDGMENTS

We thank Dr. Yuan Yuan for her critical reading and helpful proposal for this manuscript. This work was supported by grants from the National Nature Science Foundation of China (No.30171091 and No.30271478).

Declaration of interest The authors report no conflict of interest. The authors alone are responsible for the content and writing of this paper.