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Cancer Investigation Volume 33, 2015 - Issue 9
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ORIGINAL ARTICLE

Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach

Jingjing LvInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,;The Second Military Medical University, Shanghai, China,
,
Naijun FanInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,
,
Yangkun WangDepartment of Pathology, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China
,
Xiuli WangInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,
&
Chunfang GaoInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of Chinese People's Liberation Army, Luoyang, China,Correspondence[email protected]
Pages 420-428 | Received 01 Jan 2015, Accepted 11 May 2015, Published online: 16 Jul 2015
 
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Abstract

Liquid chromatograph-mass spectrometer (LC/MS) based labeled with isobaric mass tags for relative and absolute quantitation (iTRAQ) analyses were performed to identify differentially expressed proteins from normal and cancerous human colorectal tissues. Around 802 proteins were identified, 68 proteins of which were defined as differentially expressed proteins. Bioinformatics analysis indicated that these differentially expressed proteins correlated with several specific cellular processes and pathways which have relationships with pathological changes of colorectal cancer (CRC). EHD2 were selected to verify its expression patterns and localization using western blotting and immunohistochemistry respectively. LC/MS-based iTRAQ proteomic approach would provide new information about the character of CRC.

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