Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges

Abstract

Cross-linked enzyme aggregate (CLEA) technology has been regarded as an effective carrier-free immobilization method. This method is very attractive due to its simplicity and robustness, as well as for the possibility of using the crude enzyme extract and the opportunity to co-immobilize multiple different enzymes. The resulting CLEAs generally exhibit high catalyst productivities, improved storage and operational stability and are easy to recycle. Nowadays, although the technology has been applied to various enzymes, some undesirable properties have limited its further application. To overcome these limitations, novel strategies have been developing in recent years. This mini-review focuses on process optimization, new improved strategies and the latest advances on CLEAs technology.

• Biocatalysis
• cross-linked enzyme aggregates
• enzyme immobilization
• improved strategies
• process optimization

Declaration of interest

The Project Partially Supported by the National Natural Science Foundation of China (NSFC, Project No. 21072041), the Foundation (NO. 2012IM004) of Tianjin Key Laboratory of Industrial Microbiology (Tianjin University of Science and Technology), P. R. China, and Foundation of Hebei University of Science and technology for Distinguished Young Scientists.