DISSOCIATION OF THE CIRCADIAN SYSTEM OF OCTODON DEGUS BY T28 AND T21 LIGHT-DARK CYCLES

Abstract

Octodon degus is a primarily diurnal rodent that presents great variation in its circadian chronotypes due to the interaction between two phase angles of entrainment, diurnal and nocturnal, and the graded masking effects of environmental light and temperature. The aim of this study was to test whether the circadian system of this diurnal rodent can be internally dissociated by imposing cycles shorter and longer than 24 h, and to determine the influence of degus chronotypes and wheel-running availability on such dissociation. To this end, wheel-running activity and body temperature rhythms were studied in degus subjected to symmetrical light-dark (LD) cycles of T28h and T21h. The results show that both T-cycles dissociate the degus circadian system in two different components: one light-dependent component (LDC) that is influenced by the presence of light, and a second non–light-dependent component (NLDC) that free-runs with a period different from the external lighting cycle. The LDC was more evident in the nocturnal than diurnal chronotype, and also when wheel running was available. Our results show that, in addition to rats and mice, degus must be added to the list of species that show an internal dissociation in their circadian rhythms when exposed to forced desynchronization protocols. The existence of a multioscillatory circadian system having two groups of oscillators with low coupling strength may explain the flexibility of degus chronotypes. (Author correspondence: [email protected])

Keywords:

• Chronotype
• Dissociation
• Forced desynchronization
• Masking by light
• Octodon degus
• Oscillators
• Pacemaker
• T-cycles

ACKNOWLEDGMENTS

This project was funded by the Seneca Foundation (PI/05700/07), the Instituto de Salud Carlos III (RETICEF, RD06/0013/0019), and the Ministry of Education and Science (BFU2007-60658/BFI). The authors would like to express their appreciation to Ana Maria Madariaga, from the Animal Service at the University of Alicante, for providing the colony of degus.

Declaration of Interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.