Late emergence chronotypes of fruit flies Drosophila melanogaster exhibit higher accuracy of entrainment

Abstract

Inter-individual variation in phase-of-entrainment (chronotype) is widely observed in many species, but the underlying mechanisms and its consequences remain largely unexplored. In light of considerable limitations of previous studies proposing that the late chronotypes exhibit weakly stable rhythms, we employed outbred Drosophila populations exhibiting early and late emergence chronotypes to re-visit such associations. Contrary to previous reports, we observed that the late chronotypes consistently exhibit higher stability in emergence and activity–rest rhythms as compared to the early chronotypes, both under laboratory and semi-natural conditions, which is not associated with higher precision of circadian clocks, thus demonstrating the existence of genetic correlations between accuracy of entrainment and chronotype. Our results, along with the previously reported clock property differences between the early and the late emergence chronotypes highlights a possible complex interplay of clock period, phase response curve and accuracy in determining phase-of-entrainment.

• Accuracy
• activity/rest
• chronotype
• circadian
• emergence
• evolution
• stability

Acknowledgements

The authors thank A. Lakshman and N. N. Kannan for assistance with the experiments; A. Lakshman for the Matlab code used for analysis. We thank three anonymous reviewers for suggesting improvements to the manuscript. N.K.L., K.V. and V.K.S. conceived the idea and designed experiments. N.K.L. and K.V. performed the experiments, N.K.L. analysed the data, and N.K.L., K.V. and V.K.S. wrote the manuscript.

Declaration of interest

The authors declare no conflict of interest.