The authors would like to correct errors that occurred in the publication of Autoimmunity.
AID dysregulation in lupus-prone MRL/Faslpr/lpr mice increases class switch DNA recombination and promotes interchromosomal c-Myc/IgH loci translocations: Modulation by HoxC4
CLAYTON A. WHITE, J. SETH HAWKINS, EGEST J. PONE, ELLIOT S. YU, AHMED AL-QAHTANI, THACH MAI, HONG ZAN, & PAOLO CASALI
18 May 2011 [Epub ahead of print] (doi:10.3109/08916934.2011.577128)
Eleven of the 14 c-Myc/IgH junction sequences (TL01, TL02, TL6, TL10, TL13, TL10, TL30, TL38, TL42, TL158, and TL138—the second TL10, as italicized, was a mislabel of the sequence TL19) depicted in Figure 7 are correct. These 11 sequences were obtained by nested PCR using degenerate IgH switch region primers 5′-AGCTCATTCCAGCTCAGCTCAGCCT-3′ (first round PCR) and 5′-AGCTCAGCTCAGCCTARCCCAGCTC-3′ (second round PCR), rather than the 5′-TGAGGACCAGAGAGGGATAAAAGAGAA-3′ (first round PCR) and 5′-CACCCTGCTATTTCCTTGTTGCTAC-3′ (second round PCR), as stated in Materials and methods. Only degenerate primers can anneal to DNA of different S regions, thereby allowing amplification of c-Myc/IgH junctions arising from IgH breakpoints at different S regions (Potter et al., Blood 97: 260–269, 1997; Kovalchuk et al., J. Exp. Med. 204: 2989–3001, 2007). The three remaining sequences, TL-125, TL-187, and TL-162, possibly resulted from a PCR artifact involving primers 5′-TGAGGACCAGAGAGGGATAAAAGAGAA-3′ (first round PCR) and 5′-CACCCTGCTATTTCCTTGTTGCTAC-3′ (second round PCR), and, therefore, should be excluded from data analysis.