Purification and structure determination of gelatinase and collagenase inhibitors from Viola patrinii fermentation extracts

Abstract

Investigation of collagenase and gelatinase inhibitory natural components afforded two isoflavonoids. Two isoflavonoids, tectorigenin-7-O-β-D-glucoside (1) and luteolin-7-O-β-D-glucuronopyranoside (2), were isolated from ethyl acetate fraction of Viola patrinii fermentation extracts (VPFE). Of these, compounds 1 and 2 exhibited collagenase inhibitory activity (IC₅₀) at a concentration of less than 1.5 μM, and compound 2 showed gelatinases A and B inhibitory activity (IC₅₀) at 0.3 μM and 0.8 μM, respectively.

Keywords::

• Viola patrinii
• fermentation extracts
• isoflavonoids
• collagenase
• gelatinase